主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (3): 196-202.doi: 10.3969/j.issn.1006-2157.2018.03.004

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Regulation effects of different disassembled formulas of Huiyang Shengji Fang on macrophage phenotype transition*

Lin Yan, He Xiujuan, Zhao Jingxia, Xue Yan, Meng Yujiao, Li Ping#   

  1. Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital University of Medical Sciences, Beijing 100010, China
  • Received:2016-11-25 Online:2018-03-30 Published:2018-03-30
  • Supported by:
    *National Natural Science Foundation of China (No. 81403409, No. 81403270, No.81774328)

Abstract: Objective To observe the influence of Huiyang Shengji Fang(Restoring Yang and Promoting Tissue Regeneration Decoction, HYSJF) and its decomposed formulas-Yiqi Wenyang Fang(Qi-supplementing and Yang-warming Decoction, YQWYF) and Huoxue Tongluo Fang (Blood-activating and collateral-freeing Decoction, HXTLF) on macrophage phenotype inversion, and discuss the mechanism of YQWYF and HXTLF in regulating macrophage phenotype inversion during the period of chronic skin ulcer. Methods THP-1 cells were stimulated and transformed into macrophages with PMA. After stimulated with LPS and INF-γ for 48 h, the macrophages were transformed into M1 macrophages. Lovastatin (10 μmol /L) was taken as control drug. The influence of YQWYF and HXTLF on activities of macrophages was observed by using CCK-8 method, and their influence on the phagocytosis of macrophages was detected by using neutral red uptake assay (NRU). The expressions of iNOS mRNA and Arg-1 mRNA were detected by using polymerase chain reaction (PCR) after M1 macrophages stimulated with lovastatin, YQWYF and HXTLF for 24 h. The levels of supernate TNF-α, IL-6, IL-12, IL-10 and VEGF were detected by using CBA method. The level of supernate TGF-β was detected by using ELISA. Results YQWYF, in dose of 4 mg/L (low-dose YQWYF group), 0.8 mg/L (mid-dose YQWYF group) and 0.16 mg/L (high-dose YQWYF group), and HXTLF, in dose of 32 mg/L (low-dose HXTLF group), 6.4 mg/L (mid-dose HXTLF group) and 1.28 mg/L (high-dose HXTLF group) had no influence on proliferation of macrophages but improved phagocytosis of NRU of macrophages. The expression of iNOS mRNA of M1 macrophages increased significantly after stimulated by LPS and INF-γ, and decreased significantly in lovastatin group, YQWYF group and HXTLF group compared with model group (P<0.01). The expression of Arg-1 mRNA of M2 macrophages decreased significantly after stimulated by LPS and INF-γ compared with normal group, was improved significantly in lovastatin group, YQWYF group and HXTLF group compared with model group (P<0.01), and decreased significantly in YQWYF group and HXTLF group compared with lovastatin group. After M1 macrophages stimulated by above drugs for 24 h, the levels of TNF-α, IL-6 and IL-1 decreased significantly, and levels of VEGF and TGF-β increased significantly. The level of IL-10 increased significantly in high-dose YQWYF group (P<0.05), The levels of IL-10, VEGF and TGF-β had statistical difference in HXTLF group and high-dose YQWYF group compared with lovastatin group. Conclusion YQWYF and HXTLF can inhibit expression of iNOS mRNA of M1 macrophages and induce expression of Arg-1 mRNA of M2 macrophages, and can inhibit the secretion of M1 macrophage cytokine and improve secretion of M2 macrophage cytokine.

Key words: Huiyang Shengji Fang(Restoring Yang and Promoting Tissue Regeneation Deloction), supplementing qi and warming yang, activating blood and freeing collateral vessels, macrophages phenotype, human monocytic line

CLC Number: 

  • R285.5