主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (3): 210-215.doi: 10.3969/j.issn.1006-2157.2018.03.006

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Influence of Tiaozhi Tongmai Granule on blood fat, RLP-C, sICAM-1 and sVCAM-1 in ApoE-/- mice*

Li Lu1, Guo Yangzhi2, Yang Huimin1#, Qian Lichao1, Shi Cheng1, Wang shuyan3, Liao Lei4   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100070, China;
    2 Beijing Shijitan Hospital, Capital University of Medical Sciences, Beijing 100038, China;
    3 Beijing University of Chinese Medicine, Beijing 100029, China;
    4 Institute of Clinical Pharmacy, Beijing Municipal Health Bureau, Beijing 100053, China
  • Received:2017-10-09 Online:2018-03-30 Published:2018-03-30
  • Supported by:
    *National Natural Science Foundation of China (No. 81503503)

Abstract: Objective To investigate the influence of Tiaozhi Tongmai (Fat-regulating and Vessel-freeing) Granule (TZTMG) on blood fat, triglyceride-rich remnant-like particle (RLP) and cell adhesion molecule in ApoE-/- mice. Methods After established model of atherosclerosis (AS), ApoE-/- mice (n=30) were randomly divided into model group, fenofibrate group and TZTMG group (each n=10). C57BL/6J mice (n=10) were chosen into normal group. All medicinal groups were fed with high-fat diet, meanwhile were intervened with TZTMG and fenofibrate. The indexes of triglyceride-rich RLP (RLP-C) and 4 items of blood fat were detected detector after 12 weeks. The levels of serum intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) were detected by using ELISA. The aortic root was collected to prepare paraffin sections for observing morphological changes after HE staining. Results Since the 4th week, mouse weight decreased in TZTMG group and fenofibrate group compared with model group. The levels of serum RLP-C, TC, TG, HDL-C and LDL-C increased in model group, fenofibrate group and TZTMG group compared with normal group (P<0.05). The levels of serum RLP-C and TG decreased in fenofibrate group and TZTMG group compared with model group (P<0.05). The levels of ICAM-1 and VCAM-1 increased significantly in model group, fenofibrate group and TZTMG group compared with normal group (P<0.05). The levels of serum ICAM-1 and VCAM-1 decreased in fenofibrate group and TZTMG group compared with model group (P<0.05). Comparison in VCAM-1 among normal group, fenofibrate group and TZTMC group had no statistical significance. HE staining showed there were thickened aortic intima and vulnerable plaques in model group, and thickened aortic intima and stable plaques in TZTMG group. Conclusion TZTMG can decrease the expression of adhesion molecule and delay AS development through reducing RLP-C level.

Key words: blood fat, triglyceride-rich remnant-like particle, atherosclerosis, ApoE-/- mice

CLC Number: 

  • R285.5