主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (4): 294-300.doi: 10.3969/j.issn.1006-2157.2018.04.006

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Protective effects of concentrate granule and ultrafine powder of danshen on cerebral ischemia-reperfusion injury: a comparative study*

Li Ya, Gao Wenhui, Tang Minke#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2017-10-31 Online:2018-04-30 Published:2018-04-30
  • Supported by:
    National Science and Technology Major Projects for “Major New Drugs Innovation and Development”(No.2011ZX09201-201-22)

Abstract: Objective To compare the protective effects of concentrate granule and ultrafine powder of danshen (Salvia Miltiorrhizae Radix et Rhizome) on cerebral ischemia-reperfusion (CIR) injury. Methods Male ICR mice were randomly divided into sham operation group, model group, 0.45 g/(kg·d) danshen concentrate granule group and 2.25 g/(kg·d) danshen ultrafine powder group. CIR model was established by using middle cerebral artery occlusion (MCAO). Weight of mice was monitored throughout the experiment to evaluate their health status. The influence on neurological injury, movement and sensory function was evaluated by using neurologic score test, corner test and grip strength test. The effects of ultrafine powder and concentrate granule on CIR injury were compared using the following parameters: content of water in brain, infraction volume with TTC staining, and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and myeloperoxidase (MPO) in the brain by using ELISA. Results Compared with CIR model group, the grip strength and body weight improved significantly (P<0.01 or P<0.05) and contents of TNF-α and MPO decreased markedly (P<0.01 or P<0.05) in ultrafine powder group; content of water in brain decreased significantly (P<0.05) in concentrate granule group; cerebral infarction volume decreased markedly (P<0.01) in both danshen groups (P<0.01). Conclusion The danshen concentrate granule and ultrafine powder both have a protective effect on ischemia-reperfusion injury, and their efficacy is similar at the same dosage.

Key words: concentrate granule of Salvia Miltiorrhizae Radix et Rhizome, ultrafine powder of Salvia Miltiorrhizae Radix et Rhizome, cerebral ischemia, reperfusion, inflammatory cytokines, mice

CLC Number: 

  • R285.5