主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (4): 316-328.doi: 10.3969/j.issn.1006-2157.2018.04.010

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Quality representation and correlation analytical study of Semen Cassiae based on phenolic characteristic architecture of medical system*

Liu Lu1, Li Qian2, Zhou Deyong1, Sun Daohan1, Lu Dongbo1, Kong Jing1, Cai Xiufen3, Wang Menglin2, Wu Qiuhong1, Li Wenxia1, Li Sitong1, Fan Yuanyuan1, Huang Yujuan1, Feng Xin1, Shi Renbing1,4#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
    2 Capital Medical University, School of Chinese Medicine, Beijing 100069, China;
    3 Beijing Information Science and Technology University 100192, China;
    4 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 102488, China
  • Received:2017-12-20 Online:2018-04-30 Published:2018-04-30
  • Supported by:
    National Sci-Tech Plan of the 12th Five-Year Plan (No.2012BAI29B06), Scientific Projects of Beijing Municipal Commission of Education

Abstract: Objective To establish the quality representation and correlation analysis method and the content of characteristic ingredients based on spectrum of medicinal system of Juemingzi (Semen Cassiae), in order to evaluate the quality of Semen Cassiae decocting pieces effectively and accurately. Methods HPLC-PDA method was established to characterize the quality of spectrum of Semen Cassiae medicinal system architecture; quality of 11 batches of Semen Cassiae decocting pieces was characterized based on the number and chemical type of peaks on characteristic spectrum. The content of 6 characteristic index components, naphthopyrones and anthraquinones were characterized by indexes in characteristic spectrum. Correlation analysis of quality of Semen Cassiae was then performed with reference to the standard reference pieces. Results Taken as the reference substance, characteristic spectrum of batch 10 Semen Cassiae altogether contains 12 characteristic peaks, all characteristic peaks appeared on the chromatograms of 11 batches of Semen Cassiae. In 11 batches of Semen Cassiae, No.5, 11, 8, 1, 2 pieces contained higher effective index components, while No. 5, 11, 2, 9, and 7 pieces had high relevance with the reference piece (No.10). Consolidated quality characterization and correlation analysis, the quality of No. 5, 11, 8, 1, 2, 9, and 7 were excellent and dominant. Conclusion The HPLC method to analyze quality and quantity of phenolic components spectrum of 11 batches of Semen Cassiae is simple and accurate. The evaluation mode of quantity and quality and their correalation based on Semen Cassiae medical system, including systemic correlation and appilication validity, as it can provide a reliable methodological basis for the comprehensive quality evaluation of Semen Cassiae.

Key words: Semen Cassiae, phenolic, characteristic spectrum, correlation analysis, quality evaluation

CLC Number: 

  • R284.1