主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (5): 372-375.doi: 10.3969/j.issn.1006-2157.2018.05.004

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Influence of Jiyin Capsule on apoptosis pathway of ovarian caspase dependent cells in rats with peri-menopausal syndrome*

Zhao Lei1,2, Huang He2, Mu Yangna2, Yuan Yuan2, Bao Yulong2, Liang Maoxin1#
  

  1. 1 Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Liaoning Institute of Traditional Chinese Medicine, Liaoning 110034, China;
  • Received:2017-12-26 Online:2018-05-30 Published:2018-05-30
  • Supported by:
    National New Drugs Development of the Twelfth Five-year Plan (No. 2012zx09303017), National Clinical Key Specialty Construction, Clinical Chinese Pharmacology Key Discipline of the State Administration of Traditional Chinese Medicine

Abstract: Objective To discuss the effective mechanism of Jiyin (Nourishing Yin) Capsule in intervening peri-menopausal syndrome. Methods Normal rats (n=10) were randomly chosen into blank group. The model of peri-menopausal syndrome was established with intraperitoneal injection of VCD once a day for 60 d. When estrous cycle was disorder, level of serum estradiol (E2) was lower than the lowest level of that in blank group and level of follicle-stimulating hormone (FSH) was higher than the highest level of that in blank group, the modeling was success. The modeled rats were randomly divided into 6 groups: model group, progynova group (PGV group), Kunbao (Female Treasure) Pill group (KBP group), and low-dose, mid-dose and high-dose Jiyin Capsule groups (each n=10). After grouping, blank group and model group were given distilled water, and other groups were given corresponding medicinals once a day for 30 d. The mRNA expressions of caspase-3, caspase-8, caspase-9 and Apaf-1 were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results Compared with blank group, mRNA expressions of caspase-3, caspase-8, caspase-9 and Apaf-1 increased significantly in model group (P<0.01). Compared with model group, mRNA expressions of caspase-3, caspase-8, caspase-9 and Apaf-1 decreased significantly in Jiyin Capsule groups. Conclusion Jiyin Capsule can inhibit cell apoptosis of ovarian through down-regulating conduction of apoptosis pathway of caspase dependent cells, which maybe one of its effective mechanisms of intervening peri-menopausal syndrome.

Key words: Jiyin (Nourishing Yin) Capsule, peri-menopausal syndrome, apoptosis pathway of caspase dependent cells, rats

CLC Number: 

  • R285.5