主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (5): 376-382.doi: 10.3969/j.issn.1006-2157.2018.05.005

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Influence of Fuzheng Tixie Souluo Fang on angiogenesis in rats with pulmonary fibrosis*

Zhang Ning1, Zheng Fengjie1, Wu Huayang2, Wang Dingnan1, Yan Yuqin3, Liu Ruolin2, Gao YuShan1, Zhang Shujing1, Su Huiping2#
  

  1. 1 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    3 Beijing Longfu Hospital, Beijing 100010, China;
  • Received:2017-12-22 Online:2018-05-30 Published:2018-05-30
  • Supported by:
    National Natural Science Foundation of China (No. 81473657)

Abstract: Objective To study the influence of Fuzheng Tixie Souluo Fang (Decoction of reinforcing healthy qi, eliminating pathogen and Searching collaterals, FTSF) on angiogenesis in rats with pulmonary fibrosis (PF), and investigate its latent therapeutic mechanism. Methods Health male SD rats were randomly divided into control group, model group and treatment group (each n=32). The PF model was established by intravenous injection of bleomycin via tracheal intubation in model group and treatment group, and control group was given intratracheal injection of isovolumetric normal saline. After modeling for 24 h, treatment group was given orally suspension of FTSF [0.67g/(kg·d)], and control group and model group were given isovolumetric normal saline. All rats were executed respectively at different time points (7 d, 14 d, 21 d and 28 d, 8 each time) after modeling. The lung tissue was collected and given HE and Masson staining for detecting the expressions of vascular endothelial growth factor-A (VEGF-A) and its receptor-VEGFR-2 by using ELISA. The expression of CD34 antigen in lung tissue was detected by using immunohistochemistry (IHC) staining, and micro-vessel density was measured by using Weidner method. Results The pathological changes of lung tissue in treatment group were similar to those in model group, but severity of inflammation and fibrosis was milder than those in model group at the same time point. The expressions of VEGF-A and VEGF-R2 were lower in treatment group than those in model group at the same time point (P<0.01 or P<0.05). The staining of CD34 positive cells showed pale brown or brown, and micro-vessel density was lower in treatment group than that in model group (P<0.01 or P<0.05). Conclusion FTSF can relieve inflammatory exudation and collagen deposition of lung tissue induced by bleomycin in PF rats. The mechanism may be related to the decreases of VEGF-A and VEGF-R2 expressions and inhibition of abnormal angiogenic activity in lung tissue.

Key words: Fuzheng Tixie Souluo Fang (Decoction of reinforcing healthy qi, eliminating pathogen and Searching collaterals), pulmonary fibrosis, angiogenesis, rats

CLC Number: 

  • R285.5