主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (5): 400-404.doi: 10.3969/j.issn.1006-2157.2018.05.008

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Quantitative determination of N-caffeoylputrescine, kukoamine B and chlorogenic acid in Digupi*

Su Lei, Jiang Yanyan, Chen Luxiao, Liu Jing, Liu Bin#
  

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
  • Received:2017-12-20 Online:2018-05-30 Published:2018-05-30
  • Supported by:
    National Natural Science Foundation of China (No.81173520)

Abstract: Objective To establish a method for quantitative determination of N-caffeoylputrescine, kukoamine B and chlorogenic acid in Digupi (Chinese Wolfberry Root-bark, Cortex Lycii). Methods The preparation method for solution of test sample was established by using single-factor investigation. HPLC procedure was performed for determining the content of N-caffeoylputrescine, kukoamine B and chlorogenic acid on chromatographic column of Waters Xbridge C18 (4.6 mm×250 mm, 5 μm), mobile phase was methanol-0.05% trifluoroacetic acid in gradient elution. The flow velocity was 1.0 mL/min and the detection wavelength was 290 nm at 30℃. Results The solution of test sample was prepared after Digupi was extracted with 100 times amount of 70% methanol by using reflux extraction for 60 min. N-caffeoylputrescine showed a good linear relationship at a range from 0.013 75 mg/mL to 0.220 0 g/L (r=0.999 5), and average recovery was 99.25% (RSD=1.81%, n=6). Kukoamine B showed a good linear relationship at a range from 0.057 875 g/L to 0.926 0 mg/mL (r=0.999 8), and average recovery was 101.01% (RSD=2.81%, n=6). Chlorogenic acid showed a good linear relationship at a range from 0.009 25 g/L to 0.148 0 g/L (r=0.999 5), and average recovery was 99.51% (RSD=2.32%, n=6). Conclusion The established quantitative determination method is easy and accurate with a good repeatability, which can lay a foundation for the quality control and reviewing of Digupi.

Key words: Digupi (Chinese Wolfberry Root-bark, Cortex Lycii), high performance liquid chromatograph, quantitative determination, N-caffeoylputrescine, kukoamine B

CLC Number: 

  • R284.1