主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (6): 471-475.doi: 10.3969/j.issn.1006-2157.2018.06.006

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Effects of modified biejiajian pill on the proliferation and apoptosis of hepatocellular carcinoma HCCLM3 cells*

Gao Yebo1,2, Li Weidong1, Liu Rui1, Guo Qiujun1, Jiang Shulong1,3#   

  1. 1 Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China;
    2 Department of Oncology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China;
    3 Department of Oncology, Ji’ning No.1 People's Hospital, Shandong 272111, China
  • Received:2018-08-02 Online:2018-06-30 Published:2018-06-30
  • Supported by:
    National Natural Science of China (No. 81403346), China Postdoctoral Science Foundation Project (No. 2014M550132), Shandong National Science Foundation Project (No. ZR2014HL095)

Abstract: Objective To investigate the effects of modified biejiajian(Turtle Shell Decoction, BJJ) pill on the proliferation and apoptosis of hepatocellular carcinoma HCCLM3 cells. Methods Modified BJJ pill was orally administered to SD rats at 21 g/kg dosage for 7 days. Blood samples were drawn from the rat abdominal aorta at d7 and then centrifuged to prepare medicated serum. Modified BJJ medicated serum at different concentrations were added into 96-well-plates. At 24 h, 48 h, 72 h, 10 μL MTT solution was further added into each well, incubated for another 4h, and effects of medicated serum on the proliferation of HCCLM3 were then observed; Cultured HCCLM3 cells were administered with Chinese medicine(CM) medicated serum (5%,10%,20%) and cucurbitacin I, then the cells were incubated for 24h and 48h, on which Annexin V-FITC/PI was used to evaluate the effects of medicated serum on HCCLM3 apoptosis. Results At 24h after treated with BJJ at different concentrations, 5% and 10% of BJJ had little effect on HCCLM3 cell proliferation, and the difference was not statistically significant compared with the control group (P>0.05). After 48-h treatment of BJJ, cell proliferation rates were significantly higher in the BJJ and BJJ groups (87.86%, 84.60% respectively) compared with the control group (P<0.05). 5% BJJ had little effect on HCCLM3 cell apoptosis, and the difference was not statistically significant (P>0.05) compared with control group. Apoptosis rates were 7.90%, 12.22% and 15.36% in 10% BJJ, 20% BJJ and cucurbitacin I (0.5μmol/l) groups respectively with significant difference compared with the control group (P<0.05). After 48-h treatment, apoptosis rates in the 5%,10%,and 20% BJJ, and cucurbitacin I (0.5μmol/l) groups were 8.08%, 11.95%, 13.89% and 19.02% respectively, statistically higher than that in the control group (P<0.05). Conclusion s Modified BJJ medicated serum could inhibit the proliferation and increase the apoptosis rates of HCCLM3 cells. The effects were dose-dependent.

Key words: biejiajian pill, hepatocellular carcinoma, HCCLM3 cell, proliferation, apoptosis

CLC Number: 

  • R285.5