主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (6): 476-481.doi: 10.3969/j.issn.1006-2157.2018.06.007

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Effects of curcumin on the expression of microRNA-21 and fibrosis-related factors in rat renal interstitial fibroblast cells*

Liu Yi1, Wang Xiting1, Zhang Lan1, Sun Xuejiao1, Han Sen1, Tang Lei2, Li Yadong2, Li Yu1#   

  1. 1 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 School of Life Science, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2018-01-09 Online:2018-06-30 Published:2018-06-30
  • Supported by:
    National Natural Science Foundation of China (No. 81573716)

Abstract: Objective To investigate the effects of curcumin on expression of microRNA-21 and fibrosis-related factors induced by transforming growth factor-beta 1(TGF-β1) in the renal interstitial fibroblast cells (NRK49F) of rats. Methods NRK49F cells were divided into normal group, model group, and curcumin groups. NRK49F cells in the model group were activated by TGF-β1(10μg/L). Cells in the curcumin groups were treated with curcumin at high, medium, and low concentration(20,10,5μmol/L respectively) for 24 and 72 hours. The protein expression of α-SMA were measured by using immunofluorescence cell staining and cell analyzer. The expression of micro RNA-21 and mRNA of fibrosis-related factors (COL1A1, COL1A2, COL3A1, ACTA2, Smad3) were measured with RT-PCR. Results After treated with TGF-β1 for 72 hours, the protein expression level of α-SMA increased significantly(P<0.01);Compared with TGF-β1-treated group, the protein expression level of α-SMA decreased significantly in the curcumin groups (20,10,5 μmol/L)(P<0.01, P<0.01, P<0.05). After treated with TGF-β1 for 24 hours, the expression level of miR-21, COL1A1, COL3A1, α-SMA, and Smad3 mRNA increased significantly (P<0.01). Compared with TGF-β1-treated group, the expression level of miR-21, COL1a1, COL3a1, α-SMA, and Smad3 mRNA decreased significantly in the curcumin groups (20,10 μmol/L)(P<0.01, P<0.05);Compared with TGF-β1-treated group, the expression level of COL1A1, COL3A1, and α-SMA mRNA decreased significantly (P<0.01) and the expression level of miR-21 and smad3 mRNA showed a downward trend in the 5 μmol/L curcumin group. Conclusion Curcumin down-regulated fibrosis-related gene and protein expression induced by TGF-β1 in rat renal interstitial fibroblasts, which may be related to its down-regulating the expression of miR-21 genes.

Key words: microRNA-21, curcumin, renal fibrosis, TGF-β1, rat renal interstitial fitroblast cells

CLC Number: 

  • R285.5