主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (6): 490-496.doi: 10.3969/j.issn.1006-2157.2018.06.009

Previous Articles     Next Articles

Effects of Yi’nao Jieyu formula on magnetic resonance spectroscopy of rats with post-stroke anxiety*

Zhang Yuan1,2, Zhao Ruizhen1, Zhao Zijun1,2, Tang Qisheng1#   

  1. 1 Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2017-12-18 Online:2018-06-30 Published:2018-06-30
  • Supported by:
    National Natural Science Foundationof China(No.81473658), Specialized Research Fund for the Doctoral Program of Higher Education (No.20130013130002), Specialized Research Project sponsored by China State Administration of Traditional Chinese Medicine (No.201507001-12)

Abstract: Objective To observe metabolism changes in rats with post-stroke anxiety (PSA) and effects of Yi’nao Jieyu(brain-benefiting depression-resolving, YNJY)formula by using magnetic resonance spectroscopy. Methods Wistar rats were randomly divided into normal group, model group, sham-operation group, and YNJY group. PSA model was established by injecting collagenase (type VII) into the right globus pallidus combined with empty water bottle stimulation. Saline was injected instead of collagenase in rats of the sham-operation group. Rats in the YNJY group received YNJY formula (4.19 g/kg) for 4 weeks afterwards while those in the normal, model, and sham-operation groups were given distilled water (10 mL/g). Morphology and behavior (elevated plus maze) were observed after 4 weeks. Magnetic resonance spectroscopy was utilized at week 4 as well. Magnetic resonance spectroscopy imaging was the last one to be detected. Bilateral prefrontal cortex and hippocampus were scanned in each rat and the relative contents of N-acetylaspartic acid (NAA), choline-containing compounds (Cho), myoinositol(mI), glutamate (Glu) and creatine (Cr) were recorded. Results 1. Morphology: Compared with the normal group, rats in the model group ate less, hadmessy and dull fur, behaved aggressively, and gnawed the cage frequently. Compared with the model group, rats in the YNJY group had less aggressive behaviors, and their fur appeared softer and smoother. 2. Elevated plus maze: entry into and duration in open arms were statistically significantly less in the model group than the normal and YNJY group(P<0.05). 3. Magnetic resonance spectroscopy: model rats had lower level of NAA/Cr and Cho/Cr, and higher level of Glu/Cr in the right pre-frontal cortex than normal rats (P<0.05, P<0.01), but there was no significant differences in left pre-frontal cortex and both hippocampi (P>0.05). Compared with the model group, levels of NAA/Cr and Cho/Cr were higher in the YNJY group, while Glu/Cr level was lower in the right pre-frontal cortex (P<0.05). Conclusion YNJY formula could restore cerebral metabolism caused by PSA, relieve the post-stroke anxiety, the mechanism of which may be related with reducing amino acid neurotransmitters and repairing of impaired neurons.

Key words: Yi’nao Jieyu formula, stroke, anxiety, magnetic resonance imaging, cerebral metabolism, rats

CLC Number: 

  • R285.5