主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (6): 502-507.doi: 10.3969/j.issn.1006-2157.2018.06.011

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Simultaneous determining three active components of qi-boosting and pulse-restoring granule with HPLC*

Zhang Ziwei1, Wang Min1, Tan Jiawei1,Cui Yahua1, Zhang Qiang1, Jia Shan1, Du Shouying1#, Bai Jie1, Ma Junming1, Wan Jie2   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
    2 Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2017-12-28 Online:2018-06-30 Published:2018-06-30
  • Supported by:
    Beijing Science and Technology Plan Project(No.Z141100002214005) Beijing Municipal Young Talents Key Project(No.2016000020124G076)

Abstract: Objective To establish a method for simultaneous determination of paeoniflorin, salvianolic acid B and tanshinoneⅡA in yiqi fumai keli (qi-boosting and pulse-restoring, YQFM) granule. Methods The contents of paeoniflorin, salvianolic acid B and tanshinoneⅡA were determined by using HPLC method as Hypersil Gold Aq RPC18 column (4.6mm × 250mm, 5 μm) was utilized; the mobile phase was gradient-eluted by acetonitrile-0.05%phosphoric acid. The flow rate was 1.3 mL/min. As for the detection wave length, 230nm was set for Paeoniflorin,286 nm for salvianolic acid B and 270 nm for tanshinoneⅡA.The column temperature was 30℃and the sample injection’s volume was 10 μL. Results Paeoniflorin showed a good linear relationship with the peak at the range of 24.36~507.42 mg/L and the rvalue was 0.999 1. Salvianolic acid B showed a good linear relationship with the peak at the range of 17.40 to 362.48 mg/L and the rvalue was 0.999 8. TanshinoneⅡA showed a good linear relationship with the peak area at the range of 0.97 to 20.18 mg/L andthe r value was 0.999 9. The average recovery rates of the three components were all between 95% and 105% and the RSD values were all less than 3.00%. Conclusion The method seems accurate,specific, sensitive, and can be used for the quality control of qi-boosting and pulse-restoring granule.

Key words: paeoniflorin, salvianolic acid B, tanshinone ⅡA, qi-boosting and pulse-restoring granule, HPLC

CLC Number: 

  • R284.1