主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (7): 547-552.doi: 10.3969/j.issn.1006-2157.2018.07.004

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Influence of Jiawei Shaoyao Gancao Tang on proliferation of adenomyosis cells through regulating P53-273H*

Jiang Xinchan1, Li Kunyin2#, Guan Yongge3, Wang Shuai3, Guo Yudan4   

  1. 1 Guangdong Pharmaceutical University, Guangdong 510006, China;
    2 Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong 510360, China;
    3 First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong 510000, China;
    4 Guangzhou University of Chinese Medicine, Guangdong 510000, China
  • Received:2017-12-27 Online:2018-07-30 Published:2018-07-30
  • Supported by:
    General Program National Natural Science Foundation of China(No.81473715)

Abstract: Objective To study the influence of Jiawei Shaoyao Gancao Tang (Modified Debark Peony Root and Liquorice Root Decoction, JSGT) on proliferation of adenomyosis(AM) cells through regulating P53-273H. Methods The human AM cells were collected and cultured, and then divided randomly into JSGT group, mifepristone group and blank group. After treatment with corresponding medicinals for 24 h and 48 h, the influence of JSGT on apoptosis of AM focus cells was detected by using flow cytometry (FCM). The expressions of P53-273H mRNA and LIFR mRNA of AM cells were detected by using real-time reverse transcription quantitative polymerase chain reaction (RT-PCR). The protein expressions of P53-273H, LIF, STAT3 and p-STAT3 were detected by using Western blotting assay. Results The results of FCM showed that the apoptosis rate was significantly higher in JSGT group than that in blank group (P<0.01). The results of real-time RT-PCR showed that the relative expressions of P53-273H mRNA and LIFR mRNA were lower in JSGT group than those in blank group (P<0.05). The results of Western blotting assay showed that, after intervention with JSGT for 24 h and 48 h, the protein expressions of P53-273H, LIF, STAT3 and p-STAT3 were lower in JSGT group than those in mifepristone group (P<0.05) and blank group (P<0.05). Conclusion JSGT can inhibit the proliferation and improve the apoptosis of AM cells, and the mechanism may be related to that JSGT can regulate P53/LIF/STAT3 signal pathway.

Key words: adenomyosis, Jiawei Shaoyao Gancao Tang (Modified Debark Peony Root and Liquorice Root Decoction), apoptosis

CLC Number: 

  • R285.5