主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (7): 579-584.doi: 10.3969/j.issn.1006-2157.2018.07.009

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Changes of cAMP-PKA signal transduction pathways in small intestinal tissue between spleen qi deficiency pattern and kidney qi deficiency pattern in rats*

Lyu Xiaoming1,2, Song Nan1,2, Wang Deshan3, Shan Dehong3, Yang Guanlin1,2#, Jia Lianqun1,2   

  1. 1 Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Center for Translational Medicine Research of Traditional Chinese Medicine in Liaoning Province, Liaoning 110847, China;
    3 Fundamental Medical College of Liaoning University of Traditional Chinese Medicine , Liaoning 110847, China
  • Received:2018-01-17 Online:2018-07-30 Published:2018-07-30
  • Supported by:
    National Key Basic Research Development Plan (“973”Project, No. 2013CB531700), National Natural Science Foundation of China (No. 81774022), Liaoning Talents Project Funding Project (No. 2017-58)

Abstract: Objective To compare the changes of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signal transduction pathways in ileum tissue between spleen qi deficiency pattern and kidney qi deficiency pattern in rats. Methods Male SD rats (n=24) were randomly divided into normal group, spleen qi deficiency group and kidney qi deficiency group. The model of spleen qi deficiency pattern was established by repletion for 1 d, fasting for 2 d and swimming for 15 d, and model of kidney qi deficiency pattern, by intraperitoneal injection of kanamycin (250 mg/kg) twice a day for 14 d. Taking food and drinking water were freely for all rats. The activity of cAMP in ileum tissue was detected by using ELISA in all groups after modeling. The mRNA and protein expressions of PKA, glycogen phosphorylase (GP) and phosphorylase kinase (PHK) were detected by using RT-PCR and Western blotting assay. Results The content of cAMP decreased significantly in spleen qi deficiency group and kidney qi deficiency group compared with normal group (P<0.05 or P<0.01), and decreased more in spleen qi deficiency group (P<0.01). The mRNA and protein expressions of PKA decreased significantly in spleen qi deficiency group (P<0.01) and protein expressions of PKA decreased in kidney qi deficiency group (P<0.05) compared with normal group. Compared with normal group, the mRNA and protein expressions of PHK decreased significantly in spleen qi deficiency group (P<0.01) and protein expressions of PHK decreased in kidney qi deficiency group (P<0.05). The mRNA expression of PHK was higher in kidney qi deficiency group than that in spleen qi deficiency group (P<0.05). The mRNA and protein expressions of GP decreased significantly in spleen qi deficiency group (P<0.01), and mRNA expression of GP decreased in kidney qi deficiency group (P<0.01) compared with normal group. The mRNA and protein expressions of GP were higher in kidney qi deficiency group than those in spleen qi deficiency group (P<0.01). Conclusion The changes of cAMP-PKA signal transduction pathways have difference between spleen qi deficiency pattern and kidney qi deficiency pattern in rats. As to cAMP content, it decreases more in spleen qi deficiency group compared with kidney qi deficiency group, it can be deduced that low energy performances (lack of strength, etc.) are more serious in spleen qi deficiency pattern.

Key words: spleen qi deficiency pattern, kidney qi deficiency pattern, cyclic adenosine monophosphate-protein kinase A, signal transduction, rats

CLC Number: 

  • R285.5