主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (7): 593-598.doi: 10.3969/j.issn.1006-2157.2018.07.011

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Simultaneous determination of 6 components in serum from Mongolian medicine compound formula-Shudage-4 by using HPLC*

Xin Shasha, Ji Zengyun, Du Yinfei, Dong Yu#   

  1. School of Pharmacy, Inner Mongolia Medical University, Inner Mongolia 010110, China
  • Received:2017-12-18 Online:2018-07-30 Published:2018-07-30
  • Supported by:
    National Natural Science Foundation of China (No. 81260680), Project of Youth Science and Technology Leading Talent of Inner Mongolia in 2014 (No. NJYT-14-A12), Project of Science and Technology Innovation Guide Award Fund of Inner Mongolia in 2016

Abstract: Objective To establish a method for simultaneously determining the content of 1-phenyl-7-(3-methoxy-4-hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3- methylether, α-asarone and costunolide in serum from Mongolian medicine compound formula-Shudage-4 by using HPLC. Methods The HPLC procedure was performed on the chromatographic column of Agilent C18 (4.6 mm×250 mm, 5 μm) at 30℃, and mobile phase was acetonitrile (A)-0.2% water solution of acetic acid (B) in gradient elution. The flow velocity was 0.6 mL/min, sample size was 100 μL and detection wavelength was 254 nm. Results The mass concentration and peak areas of 1-phenyl-7-(3-methoxy-4-hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, α-asarone and costunolide showed good linear relationships (r=0.999 0-0.999 8). The average recoveries of samples (n=6) were 91.74%- 102.10%, and RSD were 1.03%-2.61%. The average content of 1-phenyl-7-(3-methoxy-4- hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, α-asarone and costunolide was, respectively, 3.200 0 mg/L, 0.647 5 mg/L, 0.865 6 mg/L, 0.156 0 mg/L, 0.331 9 mg/L and 3.075 1 mg/L. Conclusion The method is easy and stable with higher repeatability, and it can be used in quality control of Shudage-4 and provide a basis for serum pharmacochemistry study of Shudage-4 resources.

Key words: Shudage-4, serum, high performance liquid chromatography, quantitative determination

CLC Number: 

  • R284.2