主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (8): 636-641.doi: 10.3969/j.issn.1006-2157.2018.08.004

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Protective effects of hydroxysafflor yellow A on oxidative damage in H9c2 cells through inhibiting microRNA-1*

Wu Hong1,2, Lei Zhen2, Gao Shuibo2, Zhang Lingxia3, Dai Liping3#   

  1. 1 Laboratory of Cell Imaging, Henan University of Chinese Medicine, Henan 450002, China;
    2 Second School of Clinical Medicine, Henan University of Chinese Medicine, Henan 450002, China;
    3 Henan University of Chinese Medicine, Henan 450046, China
  • Received:2018-03-05 Online:2018-08-30 Published:2018-08-30
  • Supported by:
    National Natural Science Foundation of China (No. 81473453, No.81673800), the Science & Technology Innovation Talents in Universities of Henan Province (No. 14HASTIT028), the Henan Science and Technology Project (No. 142102310040)

Abstract: Objective To investigate the protective effect of hydroxysafflor yellow A (HSYA) on oxidative damage in cardiomyocytes, and to further observe the relationship between this effect and microRNA-1 (miR-1). Methods The optimal concentration of HSYA was firstly screened by cell viability. The cells were divided into blank group, model group and HSYA group. The level of reactive oxygen species (ROS) was detected by using fluorescence microscope; the rate of apoptosis was measured with flow cytometry and the expression of miR-1 was determined by real-time q-PCR, on which the protective effect of HSYA on myocardial cells injured by H2O2 was clarified. Furthermore, the level of miR-1 and ROS in miR-1 mimic-transfecting myocardial cells injured by H2O2 was explored, as well as the effect of HSYA on the injuries. Results Compared with the blank group, the level of ROS, apoptosis rate, and miR-1 expression all increased in model group, and the differences were statistically significant. Compared with model group, the level of ROS, apoptosis rate and miR-1 expression all decreased in HSYA group, and the differences were also statistically significant. After the transfection of H9c2 cells with miR-1 mimics, the level of miR-1 and ROS increased, which was superimposed on the level of miR-1 and ROS induced by H2O2, while HSYA downregulated the level of miR-1 and ROS. Conclusion HSYA can reduce the level of ROS generation and apoptosis induced by H2O2 in H9c2 cells and defend against oxidative damage in cardiomyocytes. The mechanism may be related to down-regulation of miR-1.

Key words: hydroxysafflor yellow A, reactive oxygen species, apoptosis, miR-1, oxidative damage, H9c2 rat cardiomyocytes

CLC Number: 

  • R285.5