主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (8): 642-647.doi: 10.3969/j.issn.1006-2157.2018.08.005

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Mechanisms of Baoyuan Jiedu decoction in the intervention of carcinogenic muscular atrophy through inhibiting cytokins-ubiquitin-proteasome pathway*

Zhang Honghua1,2, Zong Xin1, Deng Tiantian1, Zhao Ruohan1, Ji Xuming 1,3#   

  1. 1 Shandong University of Traditional Chinese Medicine,Shandong 250355,China;
    2 Jining Medical College, Shandong 272067, China;
    3 School of Preclinical Medicine, Zhejiang University of Traditional Chinese Medicine, Zhejiang 310053, China
  • Received:2018-02-09 Online:2018-08-30 Published:2018-08-30
  • Supported by:
    National Natural Science Foundation of China(No.81573871, No.81273634, No.81774198, No.81703839), Key Technological Innovation Project of Key Industries in Shandong Province (No.2016CYJS08A01-3,No.2016CYJS08A01-4),Key Research and Development Plan of Shandong Province(No.2016ZDJS07A12)

Abstract: Objective To investigate the effect of Baoyuan Jiedu (BYJD) decoction on C2C12 cells differentiation in cancer-induced myotube atrophia model and explore its mechanism. Methods Lewis lung carcinoma cell supernatant at different concentrations (1∶2,1∶4, and 1∶8) were injected into C2C12 cells to induce cancer-induced myotube atrophy and identify the optimal concentration and culture duration. Four groups was divided after the model was built: normal group, high-dose BYJD group (125 g/L), mid-dose BYJD group (62.5 g/L), and low-dose BYJD group (31.25 g/L). The condition of myotube was observed under electro-microscope; levels of TNF-α and IL-6 were measured with ELISA; muscle fiber transverse diameter of C2C12 cells were observed by using inverted microscopy at every 24 h; the expressions of Atrogin-1 and MuRF-1 were detected by using Real-time PCR and Western blot assay. Results TNF-α and IL-6 in C2C12 indicated that the optimal condition to induce cancer-induced myotube atrophy was Lewis lung carcinoma cell supernatant at concentration of 1∶2 for 96 hours. TNF-α and IL-6 in BYJD groups decreased significantly (P<0.05); transverse diameter of myotubes in BYJD groups were prolonged after intervention of BYJD decoction at high-dose, mid-dose and low-dose (P<0.05); the expression of Atrogin-1, MuRF-1 protein and mRNA was down-regulated (P<0.05). Conclusion BYJD decoction could effectively reduce cancer-induced myotube atrophy by inhibiting UPP through lowering cytokine levels.

Key words: Baoyuan Jiedu decoction, cancer cachexia, cancer-induced myotube atrophy, cytokines, ubiquitin-proteasome pathway, C2C12 cell

CLC Number: 

  • R285.5