主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (8): 648-654.doi: 10.3969/j.issn.1006-2157.2018.08.006

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Effects and mechanisms of Bushen Tiaojing Fang on the embryo implantation via STAT3 signaling pathway during implantation window in superovulated mouse*

Duan Yancang, Li Zhili, Liu Wei, Fu Tian, Yang Jiangxia, Wang Yahui, Zhang Yayao, Hao Renjuan, Yu Huiwen, Du Huilan, Xu Huazhou, Ma Huirong, Song Cuimiao#   

  1. Hebei University of Chinese Medicine, Collaborative Innovation Center of Integrated Chinese and Western Medicine on Reproductive Disease, Hebei Key Laboratory of Integrative Medicine on Liver-kidney Patterns, Hebei 050200, China
  • Received:2018-02-03 Online:2018-08-30 Published:2018-08-30
  • Supported by:
    National Natural Science Foundation of China (No.81303002), Higher Institute Science & Technology Research Project of Education Bureau, Hebei Province (No.ZD2015013) and Project of Hebei Provincial Traditional Chinese Medicine Administration (No.700201601204005)

Abstract: Objective To observe the effects of Bushen Tiaojing Fang (BSTJF, kidney-tonifying menstruation-regulating formula) on endometrium of controlled ovarian hyperstimulated (COH) mice through signal transducer and activator of transcription 3(STAT3), and to explore mechanisms of BSTJF for improving embryo implantation. Methods Sixty Kunming mice were randomly divided into the model group, BSTJF group and normal group (n=20 in each group). On the fourth day of pregnancy, 10 mice from each group were sacrificed. The levels of serum estradiol (E2) and progesterone (P) were measured by using radioimmunoassay. The expressions of endometrial leukemia inhibitory factor (LIF), phosphorylated STAT3(P-STAT3) protein were evaluated with Western blot assay; Leptin and leptin receptor (OB-R) were detected with immunohistochemistry. At eighth day when all mice were then sacrificed, the average number of blastocysts was counted. Results Compared with the normal group, serum E2 level in the model group was decreased (P<0.05), and P was increased (P<0.01); the contents of LIF and P-STAT3 protein in the endometrium decreased (P<0.01); the expressions of Leptin and OB-R in epithelial cells decreased (P<0.01); the average number of implanted embryos was also decreased (P<0.01). Compared with the model group, serum P decreased in the BSTJF group(P<0.05); the contents of LIF and P-STAT3 protein in endometrium increased (P<0.01), the expression of Leptin protein in endometrial epithelial cells increased (P<0.05). The average number of blastocysts was increased (P<0.01). Conclusion BSTJF can improve the embryo implantation of COH mice. Its mechanism might be related to regulation of relative proteins in STAT3 signal pathway in the endometrium during implantation window.

Key words: Bushen Tiaojing Fang, controlled ovarian hyperstimulation, endometrium, STAT3 signaling pathway, mice

CLC Number: 

  • R285.5