主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (9): 759-770.doi: 10.3969/j.issn.1006-2157.2018.09.010

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Influence of water decoction of Huangqi on immune function in rats with deficiency cold pattern: a study based on transcriptome sequencing technique*

Yu Wanchen, Wang Shijun#, Ji Xuming, Zhao Haijun, Zong Xin, Chen Yanjiao   

  1. Shandong University of Traditional Chinese Medicine, Shandong 250355, China
  • Received:2018-04-10 Online:2018-09-30 Published:2018-09-30
  • Supported by:
    National Key Basic Research Program of China (973 Program, No. 2013CB531803), National Natural Science Foundation of China (No. 81673852, No. 81703838, No. 81603419), Natural Science Foundation of Shandong Province (No. ZR2016HM66), Key Technology Project of Major Industry in Shandong Province (No. 2016CYJS08A01-3, 2016CYJS08A01-4), Key R&D Plan of Shandong Province (No. 2016ZDJS07A12)

Abstract: Objective To investigate the molecular mechanism of occurrence of deficiency cold pattern and therapeutic effect of water decoction of Huangqi (Milkvetch Root, Radix Astragali seu Hedysari) on deficiency cold pattern. Methods The traditional Chinese medical compound [Sheng Shigao (raw Gypsum, Gypsum Fibrosum), Longdancao (Gentian Root, Radix Gentianae), Huangbai (Amur Cork-Tree, Cortex Phellodendri), Zhimu (Common Anemarrhena Rhizome, Rhizoma Anemarrhenae), in a ratio of 2∶1.2∶1∶1.5] was intragastrically given to rats [dosage=16 g/(kg·d), and dose=4 mL/(kg·d)] for 14 d for establishing rat model of deficiency cold pattern. The modeled rats were randomly divided into model group(deficiency model) and treatment group [treated with water decoction of Huangqi, dose=5.40 g/(kg·d), each n=12] by using SPSS software, and other rats (n=12) were chosen into blank group. The expressions of immune-related genes in liver were detected by using transcriptome sequencing technique. The differentially expressed genes (DEG) were screened, and immune-related genes were annotated according to GO functional standard. The Results of transcriptome sequencing were verified by using fluorescence quantitative polymerase chain reaction (PCR). Results Compared with blank group, there were 323 differential genes screened in model group. Compared with model group, there were 333 differential genes screened in treatment group, and they mainly involved the functions of stimulus response and defense response. The Results of GO functional analysis showed that model group had down-regulated expressions of multiple immune-related genes with stimulus response and defense response as representations, and immune response function was impacted and body immunity was impaired. After treatment with water decoction of Huangqi, the immune response of body to environment was improved and body immune status was recovered and enhanced through up-regulating the expressions of genes related to stimulus response, defense response and immune response. Conclusion The molecular mechanism of occurrence of deficiency cold pattern and therapeutic effect of water decoction of Huangqi may closely related to body immune function and expressions of relative genes.

Key words: water decoction of Huangqi (Milkvetch Root, Radix Astragali seu Hedysari), deficiency cold pattern, immune function, transcriptome sequencing, rats

CLC Number: 

  • R285.5