主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (9): 771-780.doi: 10.3969/j.issn.1006-2157.2018.09.011

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Influence of surrounding-hoop method of Modified Ruyi jinhuangsan Tincture Tincture, under guidance of huchang theory, on expressions of CD68 and myeloperoxidas in rats with skin abscess*

Xu Qiang1, Liu Tingting2, Wang Wanying2, Zhu Chaojun1, Zhang Zhaohui1#   

  1. 1 Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China;
    2 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Received:2018-03-21 Online:2018-09-30 Published:2018-09-30
  • Supported by:
    National Natural Science Foundation of China(No.81273759)

Abstract: Objective To observe the influence of surrounding-hoop method of Modified Ruyi jinhuangsan Tincture Tincture, under guidance of huchang theory, on expressions of macrophage markers (CD68) and myeloperoxidas (MPO) in rats with skin abscess. Methods All rats (n=50) were randomly divided into blank group, pretreatment group, treatment group, control group and model group. Except of blank group, other 4 groups were injected with Staphylococcus aureus to establish model of skin abscess, and treated externally with, respectively, Modified Ruyi jinhuangsan Tincture Tincture, Mupirocin Ointment and physiological saline. The samples of subcutaneous tissue around abscess for 1 cm,2 cm and 3 cm were collected for making into slices after treatment for 8 d, 13 d, 18 d, 22 d and 25 d, and were given immunohistochemistry staining . The expressions, changes and influence of CD68 and MPO were detected and analyzed by using microscopy and integrated optical density (IOD) detection. Results IOD values of CD68 and MPO showed a trend of firstly ascending and then descending during the treatment, and decreased with the increase of distance from abscess center and their positive expressions weakened also. The influence of Modified Ruyi jinhuangsan Tincture Tincture was two-sided on local abscess during abscess non-formation stage and late stage of abscess formation. The expressions of CD68 and MPO were lower in pretreatment group in varying degrees than those in other groups (P<0.05 or P<0.01) at different stages and distances during abscess non-formation stage. The expressions of CD68 and MPO were higher in treatment group in varying degrees than those in other groups (P<0.05 or P<0.01) after abscess formation. Conclusion The early administration of Modified Ruyi jinhuangsan Tincture Tincture for treatment skin abscess in rats can regulate the releases of CD68 and MPO, local proinflammatory cytokines, at different stages of abscess formation, and provide a favorable condition for huchang formation.

Key words: huchang theory, surrounding-hoop method, skin abscess, macrophage markers, myeloperoxidas, rats

CLC Number: 

  • R285.5