主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (9): 787-792.doi: 10.3969/j.issn.1006-2157.2018.09.013

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Plasma metabolomic study on deficiency or excessiveness patterns of chronic recurrent ulcerative colitis based on H-nuclear magnetic resonance*

Wang Jiajie1, Cao Yun2, Li Zhongfeng3, Chen Jianxin4, Wang Liang5, Huang Jiaqin2, Fu Xin2, Zhang Zhongmian2, Li Zhihong2#   

  1. 1 Yunnan Provincial Hospital of Traditional Chinese Medicine, Yunnan 650021, China;
    2 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    3 Department of Chemistry, Capital Normal University, Beijing 100048, China;
    4 Beijing University of Chinese Medicine, Beijing 100029, China;
    5 Hospital of Traditional Chinese Medicine of Beijing Huairou District, Beijing 101400, China
  • Received:2018-05-02 Online:2018-09-30 Published:2018-09-30
  • Supported by:
    National Key Research and Development Program of China (No. 2017YFC1700106), Wu jieping Medical Foundation Funded Special Clinical Research Projects (No.320.6750.17233)

Abstract: Objective To discuss the plasma metabolomic characteristics in patients with ulcerative colitis (UC) and deficiency or excessiveness patterns. Methods UC patients (n=31, UC group) with spleen-stomach qi deficiency pattern or large intestinal dampness-heat pattern and health volunteers (n=40, control group) were chosen, and general conditions were observed in 2 groups. And UC group was divided into subgroup of large intestinal dampness-heat pattern (subgroup A) and subgroup of spleen-stomach qi deficiency pattern (subgroup B). The content of plasma metabolites was determined quantitatively by using H-nuclear magnetic resonance (1H-NMR). The multivariate statistical analysis was conducted by using principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Results There were 10 potential biomarkers screened. The levels of acetoacetate and acetone were higher, and levels of lactic acid, carnosine, alanine and isoleucine were significantly lower in UC group than those in control group. The levels of glucose, threonine and carnosine were higher, and levels of valine, alanine and glycine were lower in UC patients with large intestinal dampness-heat pattern than those in UC patients with spleen-stomach qi deficiency pattern. Conclusion There are obviously disorder of glycometabolism, amino acid metabolism and lipid metabolism in UC patients with deficiency or excessiveness patterns. The pattern differentiation and classification of UC has a metabolite basis.

Key words: ulcerative colitis, metabolomics, H-nuclear magnetic resonance, large intestinal dampness-heat pattern, spleen-stomach qi deficiency pattern

CLC Number: 

  • R259.746