主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (11): 910-917.doi: 10.3969/j.issn.1006-2157.2018.11.006

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Influence of Qingre Liangxue Fang on imbalance of Th17/Treg cells in psoriasiform mouse model induced by imiquimod and interventional effect of miR-210 agonist*

Li Nuo1, Ya Lijing2, Wang Lei1, Wang Ying1, Bai Yanping1#   

  1. 1 Department of Dermatology, China-Japan Friendship Hospital, Beijing 100029, China;
    2 Beijing Fengtai Hospital of Integrated Traditional and Western Medicine, Beijing 100072, China
  • Received:2018-04-24 Online:2018-11-30 Published:2018-11-30
  • Supported by:
    Natural Science Foundation of Beijing (No.7164296), National Natural Science Foundation of China (No. 81373636)

Abstract: Objective To observe the influence of Qingre Liangxue Fang (Heat-clearing Blood-cooling Formula, QRLXF) on imbalance of Th17/Treg cells in psoriasiform mouse model induced by imiquimod and interventional effect of miR-210 agonist (AgomiR-210). Methods Female BALB/c mice (n=42) were randomly divided into 7 groups: normal group, model group, high-dose, mid-dose and low-dose QRLXF groups (30.2 mg /kg, 15.1 mg /kg, 7.05 mg /kg), and high-dose and low-dose AgomiR-210 groups (8 nmol/L, 4 nmol/L). The dynamic changes of skin lesions were observed by using scores of psoriasis area and severity index (PASI) in psoriasiform mouse model. The pathological changes in skin lesion tissue were observed by using light microscope. The expressions of Foxp3 and RORγt were detected by using immunohistochemistry technique, and expression of miR-210 was detected by using qRT-PCR. Results The skin lesions, pathological changes and PASI scores were significantly improved by QRLXF showing dose-effect dependence, and the improvement of PASI scores was the most significant in high-dose QRLXF group compared with model group (P<0.001). The pathological Results showed that AgomiR-210 reduced the therapeutic effect of QRLXF, which was more significant in low-dose QRLXF group compared with high-dose QRLXF group (P<0.01). The Results of immunohistochemistry technique showed that QRLXF up-regulated Foxp3 expression and down-regulated RORγt expression, and AgomiR-210 down-regulated Foxp3 expression and up-regulated RORγt expression. The Results of qRT-PCR showed that QRLXF inhibited miR-210 mRNA expression compared with model group (P<0.01). AgomiR-210 up-regulated miR-210 mRNA expression compared with high-dose QRLXF group (P<0.01). Conclusion QRLXF can significantly relieve the skin lesions in psoriasiform mouse model possibly through inhibiting miR-210 expression, up-regulating Foxp3 expression, down-regulating RORγt expression, and ameliorating imbalance of Th17/Treg cells.

Key words: Qingre Liangxue Fang (Heat-clearing Blood-cooling Formula), miR-210 agonist, psoriasis, imiquimod mouse model, imbalance of Th17/Treg cells

CLC Number: 

  • R285.5