主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (11): 918-927.doi: 10.3969/j.issn.1006-2157.2018.11.007

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Improvement of cognitive ability by Jianpi Huatan Huoxue Fang and possible mechanism in rats with subclinical hypothyroidism*

Chen Wei1,2, Yang Xiao2, Zhang Fengnuan3, Shun Qunqun3, Cha Yi3, Gao Tianshu4#   

  1. 1 Key Laboratory of TCM visceral manifestation and application of Ministry of Education, Liaoning University of Traditional Chinese Medicine, Liaoning 110033, China;
    2 Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Liaoning 110032, China;
    3 Liaoning University of Traditional Chinese Medicine, Liaoning 110033, China;
    4 Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Liaoning 110033, China
  • Received:2018-01-23 Online:2018-11-30 Published:2018-11-30
  • Supported by:
    Natural Science Foundation of Liaoning Province (No. 20180551132), National Natural Science Foundation for Youths (No. 81503474); Scientific Initial Foundation for Doctors of Science and Technology Department of Liaoning Province (No. 2015011101)

Abstract: Objective To study the influence of Jianpi Huatan Huoxue Fang (Spleen-fortifying Phlegm-resolving Blood-activating Formula, JHHF) on cognitive ability in rats with subclinical hypothyroidism (SCH), and discuss possible mechanism. Methods SCH rat model was established by total thyriodectomy and postoperative hypodermic injection of L-T4 in male Wistar adult rats. The rats were randomly divided into model group, low-dose group (13.7 g/kg of JHHF), high-dose group (41.1 g/kg of JHHF), L-T4 group (5.0 μɡ/kg), low-dose+L-T4 group (13.7 g/kg/d of JHHF +5.0 μɡ/kg/d), and high-dose+L-T4 group (41.1 g/kg/d of JHHF+5.0 μɡ/kg/d), and a sham-operation group was established at the same time. The treatment duration was for 6 weeks. After 6 weeks, all groups were given Morris water maze (MWM) test, and rats were executed. The levels of serum T4 (TT4) and thyroid stimulating hormone (TSH) were detected by using respectively radioimmunoassay (RIA) and immunochemiluminometric assay (ICMA). The Nissl bodies were observed in hippocampal CA1 zone by using light microscope after Nissl staining. The level of brain-derived neurotrophic factor (BDNF) in hippocampus was detected by using ELISA. The expressions of BDNF and its receptor-TrKB were detected by using RT-qPTC, and BDNF+TrKB were detected by using double-labelling immunofluorescence. Results The level of serum TSH increased significantly (P<0.01), TT4 level had no significant changes (P>0.05), the time of locating cruise and times of space exploration decreased significantly (P<0.01), and hippocampal BDNF level and mRNA expressions of BDNF and TrKB decreased significantly (P<0.01) in model group compared with sham-operation group. The level of serum TSH decreased significantly (P<0.05), the time of locating cruise and times of space exploration increased significantly (all P<0.05), hippocampal BDNF level and mRNA expressions of BDNF and TrKB increased significantly (all P<0.05), and numbers of double positive cells of BDNF/TrKB and hippocampal Nissl bodies increased significantly (all P<0.05) in all JHHF groups compared with model group. The curative effect of JHHF was better than that of L-T4, and curative effect of JHHF combined with L-T4 was the best. Conclusion JHHF can improve learning and cognitive abilities in SCH rats, and the mechanism may be related to the regulation of thyroid function and hippocampal BDNF. The combination of JHHF and L-T4 has synergistic effect.

Key words: subclinical hypothyroidism, cognitive ability, Jianpi Huatan Huoxue Fang (Spleen-fortifying Phlegm-resolving Blood-activating Formula), brain-derived neurotrophic factor

CLC Number: 

  • R285.5