主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (11): 928-934.doi: 10.3969/j.issn.1006-2157.2018.11.008

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Influence of Qingqianliuye (leaf of Cyclocarya paliurus) on skeletal muscle inflammatory response, insulin pathway proteins and glycogen synthesis in T2DM rats*

Yao Junkai1, Gao Xuemin1, Zhang Jianjun1, Li Wei1, Fu Lu1, Wang Leilei1, Jia Lan1, Zhao Xiuting2, Wang Jingxia1#   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Infinitus (China) Company LTD., Guangdong 510665, China
  • Received:2018-07-20 Online:2018-11-30 Published:2018-11-30
  • Supported by:
    National Natural Science Foundation of China(No. 81673617)

Abstract: Objective To study the influence of Qingqianliuye (leaf of Cyclocarya paliurus) aqueous extract (CPAE) on skeletal muscle inflammatory response, insulin pathway proteins and glycogen synthesis in rats with type 2 diabetes mellitus (T2DM). Methods T2DM rat model was established by high-fat diet for 8 weeks and intraperitoneal injection of low-dose STZ (30 mg/kg). The control rats were included into normal group, and modeled rats were divided into model group, metformin group, low-dose, mid-dose and high-dose CPAE groups. The normal group and model group were given 0.9% NaCI solution, metformin group was given metformin suspension (0.3 g/kg), and CPAE groups were given, respectively, CPAE (0.25 g/kg, 0.5 g/kg and 1 g/kg) for 4 weeks. After executed rats, the levels of free fatty acid (FFA) and glycogen in skeletal muscle homogenate were detected by using chromatoptometry. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were detected by using radioimmunoassay (RIA). The content of IκB kinase (IKK), nuclear factor-κB (NF-κB), c-Jun N-terminal kinase 1 (c-JNK1), insulin receptor substrate 1 (IRS1), phosphorylated insulin receptor substrate 1 (P-IRS1), phosphatidylinositol 3-kinase (PI3K), glucose transporter 4 (GLUT4) and glycogen synthase (GS) were detected by using ELISA. Results Compared with model group, the levels of FFA, TNF-α, IL-6 and IL-1β decreased significantly in all CPAE groups (P<0.05, P<0.01). The content of IKK, NF-κB and JNK1 proteins of inflammatory pathway decreased significantly in all CPAE groups (P<0.05, P<0.01). The protein expressions of IRS1, P-IRS1, PI3K, GLUT4 and GS, and glycogen content increased significantly in all CPAE groups (P<0.05, P<0.01). Conclusion CPAE raises the content of skeletal muscle insulin pathway proteins and glycogen synthesis protein, inhibits expressions of inflammatory pathway proteins, and improves glycogen synthesis in T2DM rats. The mechanism may be related to that CPAE can reduce content of skeletal muscle pro-inflammatory factors, inhibit expressions of inflammatory pathway proteins, and relieve metabolic inflammatory response.

Key words: Qingqianliuye (leaf of Cyclocarya paliurus), type 2 diabetes mellitus, skeletal muscle inflammatory response, insulin pathway proteins, glycogen synthesis, rats

CLC Number: 

  • R285.5