主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2018, Vol. 41 ›› Issue (12): 983-987.doi: 10.3969/j.issn.1006-2157.2018.12.003

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Effects of Chaihu Dayuan decoction on the TNF-α, IL-10 and PGE2 levels in the serum and peritoneal lavage fluid of rats with Escherichia coli peritonitis*

Jiang Xin, Xu Jingnan, Gao Fei, Bai Chen, Huang Ling, Mei Chencheng, Li Yini, Liu Tiegang, Gu Xiaohong#   

  1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2018-06-01 Published:2019-01-14

Abstract: Objective To investigate the effect of Chaihu Dayuan decoction (Bupleurum Moyuan-Targeting Decoction, CHDYY) on the proinflammatory and anti-inflammatory factors in the serum and peritoneal lavage fluid of rats with Escherichia coli (E. coli) peritonitis from the perspective of membrane source (mo yuan, pleurodiaphragmatic interspace) theory. Methods 54 SD rats were randomly divided into normal group, model group and CHDYY group (n=18 with the duration of intervention for 10 days in each group). Peritonitis model was established in the model group and the CHDYY group with intraperitoneal injection of E.coli solution at 20 mL/kg. The norrnal group and the model group received no treatment while the CHDYY group was orally administered with concentrated CHDYY decoction. The body temperature and body weight of the rats were recorded every day, and the samples were collected on the 1st day, 3rd day and 10th day respectively after the intervention. Levels of tumor necrosis factor alpha (TNF-α), interleukin 10 (IL-10) and prostaglandin E2 (PGE2) in serum and peritoneal lavage fluid were measured with ELISA assay. Results Compared with the model group, the body temperature of the CHDYY decoction group decreased significantly at 1~3 hour after intervention (P<0.05), and abdominal adhesion improved significantly. On the 1st and 3rd days of the CHDYY group, the serum TNF-α concentration was significantly decreased (P<0.05), and the serum IL-10 concentration in the CHDYY group was significantly increased (P<0.05) on the 1st day, 3rd day and 10thday in the CHDYY group, and the serum PGE2 concentration in the CHDYY group was significantly increased (P<0.05). The concentration of TNF-α in the peritoneal lavage fluid on the 1st, 3rd and 10th day of the CHDYY group increased significantly (P<0.01), and the concentration of IL-10 was significantly increased (P<0.05) on the 3rd day and 10th day of the CHDYY group (P<0.05). The concentration of PGE2 in the peritoneal lavage fluid of the CHDYY group was significantly increased (P<0.05) on the 10th day. Conclusion Chaihu Dayuan decoction seems to increase the level of TNF-αin the abdominal cavity, reduce the level of serum TNF-α, increase the level of anti-inflammatory factors IL-10 and PGE2 in the serum and peritoneal lavage fluid, and restore the balance between proinflammatory and anti-inflammatory factors.

Key words: Chaihu Dayuan decoction, peritonitis, tumor necrosis factor alpha, interleukin 10, prostaglandin E2

CLC Number: 

  • R285.5