主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2018, Vol. 41 ›› Issue (12): 995-1001.doi: 10.3969/j.issn.1006-2157.2018.12.005

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Effects of Dendrobium huoshanense on fatigue and energy metabolism in mice with kidney-yin deficiency*

Hou Yan, Fei Wenting, Wang Yujie, Zhou Xue, Yue Na, Wang Linyuan, Zhang Jianjun#   

  1. Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2018-07-10 Published:2019-01-14

Abstract: Objective To explore the effects of Huoshan Shihu (Dendrobium huoshanense, HSSH) on fatigue and energy metabolism regulation in mice with kidney-yin deficiency.Methods Mice model with kidney-yin deficiency was established by gavage of thyroxine. Effects of Huoshan Shihu on mice with kidney-yin deficiency were observed. The thermotropism behavior and the weight-loaded swimming time of mice were monitored. The levels of T3 and T4 were detected by using Enzyme-linked Immunosorbent Assay (ELISA); cAMP, cGMP, LDH and CK in serum were detected with radioimmunoassay, cAMP/cGMP was thns calculated. and the Na+-K+-ATP-ase and Ca2+-Mg2+-ATP-ase in liver tissue of mice were detected with chromatometry. Results Compared with the normal animals, the time duration in high-temperature zone and the loaded-swimming time of mice in model group were significantly decreased (P<0.01). The levels of T3, T4, cAMP, and cAMP/cGMP were significantly increased(P<0.01,P<0.001). The activities of LDH were decreased(P<0.05), and CK, Na+-K+-ATP-ase and Ca2+-Mg2+-ATP-ase were increased (P<0.001). Compared with the model animals, time duration in high-temperature zone and loaded-swimming time in A (0.33 g/kg), B (1 g/kg), C (2 g/kg), and D (3 g/kg) dose of HSSH group, Tiepi Shihu (Dendrobium candidum, TPSH) group, and Liuwei Dihuang Wan (Six-Ingredient Rehmannia pill, LWDH) group increased significantly; the level of cAMP/cGMP decreased significantly (P<0.05, P<0.01); and the activities of Na+-K+-ATP-ase and Ca2+-Mg2+-ATP-ase decreased significantly (P<0.01,P<0.001). B, C, and D dose of HSSH group, TPSH group and LWDH group appeared to reduce the concentration of T3 and T4 (P<0.05, P<0.01, P<0.001). the activities of LDH were significantly increased in B and C dose of HSSH, TPSH and LWDH groups, and the levels of CK decreased (P<0.05, P<0.01, P<0.001). Conclusion With the effects of nourishing yin and clearing heat, Huoshan Shihu could regulate the energy metabolism rate, improve the loaded-swimming time, and reduce the activity of energy metabolizing enzymes and the thyroxine level of the mice with kidney-yin deficiency induced by thyroxine. Its anti-fatigue mechanism may be related to the regulation of energy metabolism enzymes. This study further proves that the property of Huoshan Shihu is cold.

Key words: Dendrobium huoshanensis, anti-fatigue effect, kidney-yin deficiency, metabolism regulation, mice

CLC Number: 

  • R285.5