主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2018, Vol. 41 ›› Issue (12): 1002-1006.doi: 10.3969/j.issn.1006-2157.2018.12.006

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Effects of Buyang Huanwu decoction on myocardial α-MHC, β-MHC, Drp1 mRNA and mitochondrial ultra structure in rats with diastolic heart failure*

WANG Zhen,LI Jiebai, DONG Xin, SHEN Xiaoxu#   

  1. Beijing University of Chinese Medicine,Beijing 100029
  • Received:2018-07-10 Published:2019-01-14

Abstract: Objective To study the effect of Buyang Huanwu decoction (Yang-supplementing and Five-returning Decoction, BYHWT) on cardiac myosin heavy chain α, β (myosin heavy chain alpha and beta,α-MHC、β-MHC) mRNA and GTP enzyme dynami-lelated protrin 1(Drp 1) mRNA expressions in rats with diaslic heart foilure(DHF). Methods 48 SD Rats were randomly divided into sham operation group and model group,and the rat model was established by using abdominal aorta constriction method in model group of DHF rats. These rats were then randomly divided into model group, metoprolol group and BYHWT group; and intragastric intervention was administered accordingly, while the sham operation group and model group were given equal amount of deionized water, all once daily. After 8 weeks of continuous drug intervention, real-time fluorescence quantitative RT-PCR was used to determine myocardial α-MHC、β-MHC and Drp1 mRNA. Myocardial ultra structure was observed under Transmission Electron Microscope(TEM) . Result Compared with sham operation group, the expression level of α-MHCmRNA in model group increased (P<0.05); compared with model group, the α-MHCmRNA expression level of metoprolo group increased (P<0.05); compared with sham operation Group, the β-MHCmRNA expression level of model group decreased (P<0.05); compared with model group, the expression level of β-MHCmRNA in BYHWT group and metoprolol group increased (P<0.05); compared with sham operation Group, the Drp1 mRNAexpression level of model group decreased (P<0.05). Compared to the sham operation group, rat mitochondria in the model group seemed to be enlarged with hollow deformity and reduced numbers. Compared to the model group, rat mitochondria in the metoprolol and BYHWT group were improved in architecture and increased in quantity. Conclusion Buyang Huanwu decoction can inhibit α-MHC’s changing into β-MHC, thus delaying myocardial remodeling and protecting myocardium tissue. At the same time, by inhibiting the isolation of Drp1to mitochondria, protecting the structure and function of mitochondria, it can maintain the normal myocardial energy metabolism in diastolic heart failure and slow down the progression of heart failure.

Key words: Buyang Huanwu Decoction, myosin heavy chain, mitochondria, diastolic heart failure, rats

CLC Number: 

  • R285.5