主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (1): 21-29.doi: 10.3969/j.issn.1006-2157.2019.01.005

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Impact of extracts of Xuanshen on liver metabolome in rat model of hyperthyroidism

Zhang Ning1, 2, Li Zihui1, Zhao Hongwei1, Pang Mu1, Ye Tao2, Liu Shumin1#   

  1. 1 Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China;
    2 First Affiliated Hospital of Guiyang University of Chinese Medicine, Guizhou 550001, China
  • Received:2018-07-14 Online:2019-01-30 Published:2019-03-01
  • Contact: Liu Shumin, male, PhD., Professor. Research direction: property theory and pharmacodynamic material basis of Chinese materia medica. E-mail: keji-liu@163.com
  • Supported by:
    National Key Basic Research Program of China (973 Program,No. 2013CB531804), National Natural Science Funds for Young Scholar (No. 81302898), Heilongjiang Province Science Foundation for Youths (No. QC2012C038), Science and Technology foundation of Heilongjiang Province Educational Committee (No. 12541744)

Abstract: Objective To investigate the effect and possible mechanism of extracts of Xuanshen (Scrophularia Root, Radix Scrophulariae) on liver metabolome in rat model of hyperthyroidism. Methods The rat liver metabolites spectrums effected by Euthyrox and Xuanshen extracts were analyzed by using ultra high performance liquid chromatography-time of-flight-mass spectrometry (UPLC-TOF-MS). The difference in metabolites spectrums was studied between blank group and model group by using orthogonal partial least squares-discriminant analysis (OPLS-DA). The potential biomarkers were found in liver through variable importance projection (VIP). The interventional effects of Xuanshen extracts were discussed through comparing the difference in content of potential biomarkers. Results There were 21 potential biomarkers found in rat liver in model group, and among them 14 metabolites were significantly called back by Xuanshen extracts. The biosynthesis of glycosylphosphatidylinositol (GPI)-anchor, and metabolisms of arachidonic acid, purine, α-linolenic acid, linoleic acid, glutathione and glycerophospholipid were significantly improved by Xuanshen extracts in Euthyrox-induced hyperthyroidism rat model. Conclusion Xuanshen extracts can play a role of treating hyperthyroidism through improving relevant liver potential biomarkers and metabolism pathways in hyperthyroidism rat model.

Key words: Xuanshen (Scrophularia Root, Radix Scrophulariae), Euthyrox, hyperthyroidism, liver metabonomics, rats

CLC Number: 

  • R285.5