主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (1): 37-43.doi: 10.3969/j.issn.1006-2157.2019.01.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Influence of Maimendong Tang on lung function and endoplasmic reticulum stress in rats with pulmonary fibrosis

Shen Mengmeng1, Nan Yanan1, Tang Lei2, Tian Ruyu3, Zhu Man4, Kang Di5, Zhang Ruiying6, Niu Jianzhao7, Li Yadong1#   

  1. 1 School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Third Hospital of Beijing Chaoyang District, Beijing 100121, China;
    3 School of Science, North China University of Science and Technology, Hebei 063000, China;
    4 Zibo Hospital of Integrated Chinese and Western Medicine, Shandong 255000, China;
    5 Huaju Healthcare Assessment Information Technology (Beijing) Co., Ltd, Beijing 100102, China;
    6 Beijing 101 Middle School, Beijing 100091, China;
    7 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2019-06-12 Online:2019-01-30 Published:2019-03-01
  • Contact: Li Yadong, male, PhD., Associated Professor, Master’s Supervisor. Research direction: systematic biology study on prevention and treatment of lung fibrosis with Chinese medicine. E-mail: lydtry@sina.com
  • Supported by:
    National Natural Science Foundation of China(No.81202787,No.81573716), Supported by the 111 Project(No.b07007)

Abstract: Objective To investigate the relative mechanism of Maimendong Tang (Ophiopogon Decoction) in amelioration of lung function and endoplasmic reticulum stress (ERS) in rats with bleomycin-induced pulmonary fibrosis. Methods Male healthy SD rats were randomly divided into normal group, model group, Maimendong Tang group 1 and Maimendong Tang group 2. The rat model of pulmonary fibrosis was established by bleomycin pulverization via tracheal intubation. On the 14th d after modeling, Maimendong Tang was intragastrically given to Maimendong Tang group 1 and Maimendong Tang group 2 twice a day for 20 d continuously. The general conditions were observed, and pathological changes of lung tissue were observed after HE and Masson staining. The forced vital capacity (FVC), 0.4 second rate (FEV 0.4/FVC), mass FVC, inspiratory resistance (Ri), expiratory resistance (Re) and dynamic pulmonary compliance (Cydn) were detected by using spirometer. The expressions of glucose regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) protein in lung tissue were analyzed by using Western blotting assay and immunohistochemistry staining technique. Results Compared with normal group, FVC, FVC/lung mass and Cydn decreased, and 0.4 second rate increased in model group. Compared with model group, FVC increased and 0.4 second rate decreased and Cydn increased in Maimendong Tang group 1 and Maimendong Tang group 2, and mass FVC increased in Maimendong Tang group 2. Pathological sections showed that there were chronic inflammatory cell infiltration in consolidation zone of lung tissue and massive collagen deposition, GRP78 and CHOP with high expressions were mainly positioned in vesicular type II alveolar epithelial cells (AECIIs). The inflammation was relieved, collagen deposition was decreased, protein expressions of GRP78 and CHOP were reduced and in Maimendong Tang group 2. Conclusion Maimendong Tang can significantly improve lung function and reduce lung interstitial collagen deposition, which may be related to regulation of protein expressions of GRP78 and CHOP in AECIIs in consolidation zone of lung tissue, relief of ERS, and recovery of normal AECIIs function.

Key words: Maimendong Tang (Ophiopogon Decoction), endoplasmic reticulum stress, type II alveolar epithelial cells, glucose regulated protein 78, C/EBP homologous protein, rats

CLC Number: 

  • R285.5