主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (1): 77-83.doi: 10.3969/j.issn.1006-2157.2019.01.013

• Chinese Medicinal Resources • Previous Articles     Next Articles

Influence of exogenous gibberellin and abscisic acid on genetic expressions of key enzymes in ethylene synthesis in Taizishen

Wei Dequn1, Zhou Tao1, Zheng Wei1, Jiang Weike1#, Xiao Chenghong1, Guo Lanping2, Zhang Chenggang1, Wang Sheng2, Li Jun1   

  1. 1 Guizhou University of Traditional Chinese Medicine, Guizhou 550002, China;
    2 Center for Chinese Medicine Resources, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2018-06-06 Online:2019-01-30 Published:2019-03-01
  • Contact: Jiang Weike, male, Master, Professor, Master's Supervisor. Research direction: resources, cultivation and quality evaluation of Chinese materia medica. E-mail: jwk_88@163. com
  • Supported by:
    National Natural Science Foundation of China (No. 81460579), Construction Project of Modern Agricultural Industry Technology System (No. CARS-21),Major Research Project on Innovation Groups of Education Departmert of Guizhou Province (No.2018022), First-class Discipline Construction Project of Guizhou Province (No. GNYL2017-008), National Normalization Project of Chinese Medicinals (No. ZYBZH-Y-ZY-45)

Abstract: Objective To discuss the influence of exogenous Gibberellin (GA3) and abscisic acid (ABA) on genetic expressions of key enzymes in ethylene synthesis in Taizishen (Pseudostillaria Root, Radix Pseudostellariae), and provide reference for intelligent use of growth regulators during cultivation of Taizishen. Methods The genes of key enzymes in ethylene synthesis of Taizishen were screened and identified from transcriptome database by using local Blast method. Hai'ershen in full-bloom stage was treated with exogenous GA3 and ABA, and samples were collected at different time points (20 d, 40 d, 50 d and 60 d. The genetic expressions of key enzymes in ethylene synthesis of Taizishen were detected by using real-time fluorescence polymerase chain reaction (RT-PCR). Results There were totally 6 genes of key enzymes in ethylene synthesis in roots of Taizishen obtained, and they were named PhACO1, PhACO2, PhACO3, PhACS1, PhACS2 and PhSAMS. GA3 and ABA could regulated the expressions of above 6 genes, and expressions of PhACO1, PhACO2, PhACS1 and PhSAMS were up-regulated after treated with GA3, and expressions of PhACO3 and PhACS2 were up-regulated after 20 d to 40 d and down-regulated after 50 d to 60 d. The expressions of genes of key enzymes in ethylene synthesis in Taizishen showed down-regulation at first and then up-regulation after treated with ABA, but different genes had different demonstrations. The expression of PhACO1 was up-regulated after 20 d to 60 d, and down-regulated after 40 d to 50 d. The expressions of PhACO2 and PhACO3 were down-regulated after 20 d to 50 d, and up-regulated after 60 d. The expression of PhACS1 was down-regulated after 20, up-regulated after 40 d to 60 d. The expressions of PhACS2 and PhSAMS were down-regulated. Conclusion GA3 and ABA may control the ethylene signaling pathway to regulate plant growth through affecting the ethylene biosynthesis of Taizishen. It is speculated that Taizishen is treated with suitable concentration of GA3 in adventitious root growth period and treated with ABA treatment in root thickening period, the yield of Taizishen will be increased.

Key words: Taizishen (Pseudostillaria Root, Radix Pseudostellariae), Gibberellin, abscisic acid, key enzymes in ethylene synthesis, genetic expressions

CLC Number: 

  • R282.2