主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (2): 138-142.doi: 10.3969/j.issn.1006-2157.2019.02.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

A co-immunoprecipitation based exploration of the interaction between SOCS1 and IRAKs and regulatory effects of Ditan Zhuyu Decoction on hepatic stress injury in rats with acute intracerebral hemorrhage

Ma Di1, Wang Wei1, Xu Xiaoying2, Zhang Xiujing1, Wang Shuai1, Zhao Haibin3#   

  1. 1 Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China;
    2 Beijing Changping Hospital of Integrated Chinese and Western Medicine, Beijing 100096, China;
    3 Beijing Universify of Chinese Medicine Dong fang Hospital,Beijing 100078,China
  • Online:2019-02-28 Published:2019-02-28
  • Contact: Zhao Haibin, Ph.D., Chief physician. President's Office, Dongfang Hospital, Beijing University of Chinese Medicine. E-mail: haibin999@126.com

Abstract: Objective To study the interaction between suppressor of cytokine signaling 1 (SOCS1) and interleukin-1 receptor-associated kinases (IRAKs) and the regulatory effects of Ditan Tongyu (phlegm-eliminating stasis-removing, DTTY) Decoction. Methods SD rats were randomly divided into cerebral hemorrhage group, DTTY group and normal group. To establish the model of rats with acute intracerebral hemorrhage (AICH), co-immunoprecipitation (CO-IP) assay was used to detect interaction between SOCS1 and IRAK-1, IRAK-2, IRAK-4 and IRAK-M; pathological staining of liver tissue in each group at different time phase was observed. Results SOCS1 interacted with IRAK-1, IRAK-2, IRAK-4 and IRAK-M, which played a protective role in AICH rats with hepatic stress injury. Meanwhile, Ditan Zhuyu Decoction appeared to affect SOCS1-IRAKs interaction. Conclusion SOCS1 can interact with IRAK-2, IRAK-4 and IRAK-M, which may inhibit the inflammatory cascade reaction in Lipopolysaccharides (LPS) cells and play a key role in liver damage protection. Ditan Tongyu Decoction can effectively improve the histopathological structure of liver tissue, which is an important way to prevent and treat the stress liver injury with cerebral hemorrhage.

Key words: co-immunoprecipitation, suppressor of cytokine signaling 1, IRAKs, Ditan Tongyu Decoction, rats