主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (3): 190-197.doi: 10.3969/j.issn.1006-2157.2019.03.004

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Influence of Shenzhilin Oral Solution on expressions of hippocampal α7-nAChRs and mGluR5 in APP/PS1 double transgenic mice*

Huang Shuaiyang1, Sheng Ning1,2, Chen Fang1,3, Ren Ying1, Yang Jinduo1, Wang Pengwen1#   

  1. 1 Education Ministry and Beijing Key Laboratory of Chinese Internal Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    2 Chinese Medicine Hospital of Zaozhuang City, Beijing University of Chinese Medicine, Shandong 277000, China;
    3 Chinese Medicine Hospital of Xinjiang, Xinjiang Medical University, Xinjiang 830000, China
  • Received:2018-10-22 Online:2019-03-30 Published:2019-04-02
  • Contact: Prof. Wang Pengwen, Ph.D., Doctoral supervisor. Dongzhimen Hospital, Beijing University of Chinese Medicine. E-mail: pw_wang@163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81573927), Scientific Research and Graduate Training Project of Beijing Municipal Commission of Education (No. 2016, No. 2017)

Abstract: Objective To discuss the influence of Shenzhilin (Ginseng, Cassia Twig and Indian Bread) Oral Solution (SZL) on expressions of hippocampal α7-nAChRs and mGluR5 (neuronal synapse signaling related proteins) in APP/PS1 double transgenic mice. Methods Male APP/PS1 double transgenic mice (3 months old) were randomly divided into 5 groups: model group, donepezil group [0.92 mg/(kg·d)], high-dose SZL group [50 g/(kg·d)], mid-dose SZL group [25 g/(kg·d)] and low-dose SZL group [12.5 g/(kg·d)]. Wild type C57BL/6J mice with the same age and background were chosen into normal group. After gavage for 3 months continuously, behavioral platform test was carried out in all groups, and then hippocampal tissue samples were collected for detecting the expressions of α7-nAChRs and mGluR5 in hippocampal CA1 zone by using Western blotting assay and immunohistochemistry technique. Results The results of Western blotting assay showed that size of protein bands of α7-nAChRs were significantly narrower and color was lighter (P<0.01), and size of protein bands of mGluR5 were wider and color was darker (P<0.01) in model group than those in normal group. Compared with model group, size of protein bands of α7-nAChRs were wider and color was darker (P<0.05 or P<0.01), and size of protein bands of mGluR5 were narrower and color was lighter (P<0.05 or P<0.01) in all medicated groups. The positive cell expressions of α7-nAChRs and mGluR5 detected by using immunohistochemistry technique were consistent with those detected by using Western blotting assay. Conclusion SZL may play an anti-dementia role through regulating the key synaptic proteins in hippocampal CA1 zone, improving synapse function for slowing down brain damage due to Alzheimer disease, and maintaining memory acquisition ability.

Key words: Alzheimer disease, synapsis, Shenzhilin (Ginseng, Cassia Twig and Indian Bread) Oral Solution, α7-nAChRs, mGluR5, mice

CLC Number: 

  • R285.5