主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (3): 205-211.doi: 10.3969/j.issn.1006-2157.2019.03.005

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Treatment of yin-pattern wound with Huiyang Shengji Ointment in diabetic rats*

Jia Xianglong, Xu Xuying#   

  1. Beijing Hospital of Traditional Chinese Medicine, Capital University of Medical Sciences, Beijing 100010, China
  • Received:2018-10-21 Online:2019-03-30 Published:2019-04-02
  • Contact: Prof. Xu Xuying, Master’s supervisor. Beijing Hospital of Traditional Chinese Medicine, Capital University of Medical Sciences. E-mail: xxying7341@126.com
  • Supported by:
    National Natural Science Foundation of China (No. 81673975, No. 81873118), Beijing Capital Science and Technology Commission “Capital Features” Project (No. Z161100000516149), Beijing Municipal Science and Technology Commission “Ten Diseases and Ten Medicinals” Project (No. Z161100001816012)

Abstract: Objective To investigate the mechanism of Huiyang Shengji (Restoring Yang and Promoting Tissue Regeneration) Ointment on healing of yin-pattern wound in diabetic rats. Methods Male Wistar rats (n=66) were chosen and 12 rats (non-conformity) were excluded, and then divided randomly into test group, model group and control group (each n=18). The control group was directly given skin resection-plastic ring embedding, and other groups were given intraperitoneal injection of STZ-intramuscular injection of hormone-skin resection-plastic ring embedding for preparing model of yin-pattern wound. The test group was treated with HSO gauzes and model group and control group were treated with Vaseline gauzes once a day. After 3 d, 7 d and 10 d, 6 rats were executed in each group, and wound healing and morphologic changes were observed. The wound tissue were collected and prepared pathological sections for observing results of HE staining and immunohistochemical staining of CD34 (microangiogenesis marker). Results The healing rate was lower in model group than that in control group (P<0.01) after 3 d, 7 d and 10 d. The difference in healing rate was not significant between test group and model group after 3 d, but was significant after 7 d and 10 d. The healing rate was higher in test group than that in model group (P<0.05). The results of HE staining showed that granulation tissue grew vigorously, fibroblasts and capillaries were abundant, collagen fibers were thickened and inflammatory cells were less in test group and control group compared with model group after 7 d and 10 d. Inflammatory cells reached the highest value after 7 d and decreased significantly after 10 d, and capillaries began to increase and collagen fibers increased continuously in model group. The IOD value of CD34 increased significantly in test group and control group after 3 d and 7 d (P<0.01), and decreased after 10 d. There was statistical difference between test group and control group but was no significant. The content of CD34 increased slowly in model group during experimental stage, and there were significant statistical difference between model group and test group (P<0.01). Conclusion Huiyang Shengji Ointment can dynamically regulate yin-pattern wound to improve wound healing in diabetic rats based on theory of yang transforming qi and yin transforming body constituent.

Key words: Huiyang Shengji (Restoring Yang and Promoting Tissue Regeneration) Ointment, diabetes, yin-pattern wound, healing

CLC Number: 

  • R285.5