主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (3): 254-259.doi: 10.3969/j.issn.1006-2157.2019.03.012

• Acupuncture & Moxibution • Previous Articles     Next Articles

Differences in effects of eye acupuncture and body acupuncture on 24 h expression of cerebral aquaporin 4 in rats with cerebral ischemia-reperfusion injury*

Wang Zhe1, Fan Lilin2, Pan Qian1, Ma Xiande1, Zhang Wei1, Zhao Jinru1, Gao Yuan1, Guan Hongquan1#   

  1. 1 School of Basic Medicine, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Department of Neurosurgery, Shengjing Hospital of China Medical University, Liaoning 110004, China
  • Received:2018-09-22 Online:2019-03-30 Published:2019-04-02
  • Contact: Prof. Guan Hongquan, Doctoral supervisor. School of Basic Medicine, Liaoning University of Traditional Chinese Medicine. E-mail: hongquanguan@sina.com
  • Supported by:
    Subject of National Key Basic Research Development Plan (No. 2007CB512702), National Natural Science Foundation of China (No. 81673925), National Natural Science Foundation of Liaoning Province (No. 201602507)

Abstract: Objective To observe the influence of eye acupuncture and body acupuncture on 24 h expression of cerebral aquaporin 4 (AQP4) in rats with cerebral ischemia-reperfusion injury (CIRI), and discuss the difference in mechanism of treating CIRI between eye acupuncture and body acupuncture. Methods SD rats (n=48) were divided into 6 groups by using random digital method: normal control group, 24 h sham-operation group, 24 h model group, 24 h eye acupuncture group, 24 h body acupuncture group and 24 h outside area group. The model of CIRI was established by using thread approach in 24 h model group, 24 h eye acupuncture group, 24 h body acupuncture group and 24 h outside area group. After making ischemia for 1 s, the thread was pulled out to make reperfusion. After 1 h and rats revived fully, the neurologic function was scored by applying ZeaLonga scoring method, and acupuncture intervention was given to rats. Then reperfusion was continued for 24 h and rats were executed. The changes of cerebral neurocyte morphology were observed by using microscope, and protein and mRNA expressions of AQP4 were detected by using Western blotting assay and RQ-PCR. Results The neurological functional deficit scores decreases significantly in eye acupuncture group compared with model group. In eye acupuncture group cerebral cortex neurons increased in volume with large cores and abundant cytoplasm, and swelling of mitochondria and rough endoplasmic reticulum were significantly relieved. The protein and mRNA expressions of AQP4 decreased significantly (P<0.05). The comparison showed that there was no difference between eye acupuncture group and the body acupuncture group. Conclusion Eye acupuncture and body acupuncture have relieving effects on 24 h CIRI, and the mechanism is related to decrease of brain AQP4 expression. The difference between eye acupuncture and body acupuncture is not significant.

Key words: eye acupuncture therapy, body acupuncture therapy, cerebral ischemia-reperfusion injury, aquaporin 4, rats

CLC Number: 

  • R245.3