主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (5): 409-415.doi: 10.3969/j.issn.1006-2157.2019.05.009

Previous Articles     Next Articles

Effect and mechanism of Huxie Huaji Ointment combined with cisplatin on apoptosis of H22 cells of liver cancer*

Du Qing, Cai Yuan#, Shen Bingbing, Li Xinliang, Xie Fangyi, Zhang Shuihan   

  1. Hunan Academy of Traditional Chinese Medicine, Hunan 410006, China
  • Received:2018-11-27 Online:2019-05-30 Published:2019-06-05
  • Contact: Cai Yuan, Master, Assistant researcher. Hunan Academy of Chinese Medicine, No. 8, Yuehua Street, Yuelu District, Changsha 410006. E-mail: tcmyuanyuan@163. com

Abstract: Objective To investigate the effect and mechanism of Huxie Huaji Ointment combined with cisplatin on apoptosis of H22 cells of liver cancer. Methods SD rats were randomly divided into blank control group and Huxie Huaji Ointment, and the blood samples were collected from abdominal aorta for preparing medicated serum of Huxie Huaji Ointment after treatment continuously for 3 d. The logarithmic H22 cells were randomly divided into 5 groups: normal group, blank control group, cisplatin group, 20% of medicated serum group and 20% of medicated serum+cisplatin group. The influence of the medicated serum on H22 apoptosis was detected by using CCK-8 method, and apoptotic morphologic changes of H22 cells was observed after Hoechst 33258 staining in all groups. The expressions of B-cell lymphoma-2 (Bcl-2), cytochrome C (Cyt C), BCL2-associated X (Bax) and cysteinyl aspartate specific proteinase-9 (caspase-9) in H22 cells were detected by using high-content cell imaging analysis technology. Results Compared with blank control group, the proliferation of H22 cells was inhibited in a dose-time-dependent manner in medicated serum group,and the efficacr was the best in 20% of medicated serum group. Compared with blank control group, cisplatin group and medicated serum group, H22 cells showed nuclear chromatin aggregation and karyopyknosis in varying degrees, and more typical apoptotic characteristics in medicated serum+cisplatin group. The mean fluorescence intensity values of Cyt C, Bax and caspase-9 increased significantly in H22 cells (P<0.01 of P<0.05), and their protein expressions increased significantly. The mean fluorescence intensity value of Bcl-2 decreased significantly in H22 cells (P<0.01 of P<0.05), and its protein expression decreased significantly. Conclusion The medicated serum of Huxie Huaji Ointment combined with cisplatin can significantly inhibit the proliferation and induce the apoptosis of H22 cells, and the mechanism may be related to the imbalance of Bax and Bcl-2 protein expressions, and increases of Cyt C secretion and caspase-9 protein expression.

Key words: Huxie Huaji Ointment, H22 cells of liver cancer, apoptosis, Bcl-2, cytochrome C, Bax, cysteinyl aspartate specific proteinase-9

CLC Number: 

  • R285.5