主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (5): 416-422.doi: 10.3969/j.issn.1006-2157.2019.05.010

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Protective effect of Mongolian medicinal Xieri GA-4 in rat model of kidney injury*

Li Binxin1,2, Ji Zengyun1,2, Dong Yu1,2#, Bai Feng1,2, Zhu Xiaoling2,3, Sun Guoyuan2,3, Wang Yuexuan4   

  1. 1 College of Pharmacy, Inner Mongolian Medical University, Inner Mongolia 010110, China;
    2 Inner Mongolia Engineering Research Center for Effective Substances and Quality Control of Traditional Mongolian Medicine, Inner Mongolia 010110, China;
    3 International Hospital of Mongolian Medicine, Inner Mongolia 010065, China;
    4 Second Middle School of Hohhot City, Inner Mongolia 010070, China
  • Received:2019-01-07 Online:2019-05-30 Published:2019-06-05
  • Contact: Prof. Dong Yu, Ph. D., Doctoral supervisor. College of Pharmacy, Inner Mongolia Medical University, Jinshan Development Zone, Hohhot 010110. E-mail: dongyu010@126.com

Abstract: Objective To study the protective effect of Mongolian medicinal Xieri GA-4 in rat model of acute kidney injury induced by gentamicin. Methods Male healthy Wistar rats were randomly divided into blank group, model group, low-dose, mid-dose, high-dose Xieri GA-4 groups, group of Shenyan Xiaozhong Pian (Nephritis Swelling-relieving Tablet, SYXZ Tablet group) and prednison group. Except of blank group, other groups were given hypodermic injection of gentamicin (80 mg/kg·d) for reducing acute kidney injury for 6 d, and were treated with corresponding medicianls after 2 h for 14 d. The levels of serum creatinine (SCr), blood urea nitrogen (BUN), urine protein (UP), uracil-N-glycosylase (NAG) and kidney injury molecular (KIM-1) were detected by using ELISA after treatment. The kidney index (KI) and prostatic index (PI) were calculated in all groups. The histopathological changes of kidney tissue were observed with light microscope after HE staining. Results Compared with blank group, the urine volume(P<0.01), and levels of Scr, BUN, UP, NAG and KIM-1 (P<0.01) increased, and expressions of KI and PI (P<0.01) also increased in model group. Compared with model group, the levels of Scr, BUN and UP and PI decreased quickly(P<0.01), and expressions of NAG and KIM-1 were reduced after medicinal reaching to certain dose after continuous administration in low-dose Xieri GA-4 group(P<0.01). The urine volume, and levels of Scr, BUN, UP, NAG and KIM-1(P<0.05, P<0.01), and expressions of KI and PI decreased in mid-dose and high-dose Xieri GA-4 groups(P<0.05, P<0.01). The levels of some factors decreased in prednison group and SYXZ Tablet group in a period, but the results were better in all Xieri GA-4 groups during the whole experimental course. The proliferation of mesangial cells was relieved and tubular desquamation was significantly inhibited in low-dose, mid-dose, high-dose Xieri GA-4 groups. Conclusion Xieri GA-4 has a protective effect on rat model of kidney injury induced by gentamicin, and has therapeutic effect on PI increase induced by kidney injury.

Key words: Xieri GA-4, gentamicin, rat model of kidney injury, prostatic index, protective effect

CLC Number: 

  • R285.5