主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (5): 423-429.doi: 10.3969/j.issn.1006-2157.2019.05.011

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Controlling mechanisms of Qishan Fang based on metabonomics in mouse model of lung cancer*

Sui Yutong, Chi Wencheng, Jiang Jiakang#   

  1. First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
  • Received:2018-12-19 Online:2019-05-30 Published:2019-06-05
  • Contact: Prof. Jiang Jiakang, Ph. D., Chief Physician, Master’s Supervisor. The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, No. 24, Heping Road, Harbin 150040. E-mail: hljzysyt@126.com

Abstract: Objective To study the changes of endogenous metabolites in mouse model of lung cancer after intervention by Qishan Fang (Milkvetch Root and Taxus chinensis Formula), and investigate the metabolites and metabolic pathway related closely to lung cancer, and therapeutic effect of Qishan Fang. Methods The mouse model of Lewis lung cancer was established and all mice were randomly divided into Qishan Fang group, model group and blank group. The blood samples of all groups were given metabonomics analysis by using UPLC-HDMS technology platform. The mass spectrometry metabolic profiling was analyzed by using Ezionfo software. The potential metabolic biomarkers related to lung cancer were analyzed through OPLS-DA combined with retrieving of HMDB and KEGG and Progenesis QI software for compound identification. The influence of Qishan Fang on blood metabolic profiling and biomarkers were further studied, and mechanism of Qishan Fang in intervening blood metabonomics in Lewis mouse model of lung cancer. Results There were 22 metabolic biomarkers may related to lung cancer. It was found that Qishan Fang had significantly callback effect on 18 metabolic biomarkers related to lung cancer. The identified biomarkers with callback effects were further given MetPA analysis and 7 metabolic pathways were obtained, including pyrimidine metabolism, biosynthesis of unsaturated fatty acids, selenoamino acid metabolism, pentose phosphate pathway, glutathione metabolism, cysteine and methionine metabolism, and glycine serine and threonine metabolism. Conclusion It was found that has some therapeutic effects on lung cancer through regulating metabolic biomarkers related to lung cancer based on metabonomics research method.

Key words: Qishan Fang (Milkvetch Root and Taxus chinensis Formula), metabonomics, lung cancer, metabolic pathway, mice

CLC Number: 

  • R285.5