主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (6): 477-482.doi: 10.3969/j.issn.1006-2157.2019.06.008

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Mimenghua Granule on IL-12 and IL-6 in lacrimal gland cells of castrated male rabbits with induced dry eyes*

Jiang Pengfei1,3, Peng Jun2,3, Peng Qinghua1,2,3#   

  1. 1 Hunan University of Chinese Medicine, Hunan 410208, China;
    2 First Hospital of Hunan University of Chinese Medicine, Hunan 410007, China;
    3 Key Laboratory of Hunan Province for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases with Traditional Chinese Medicine, Hunan 410208, China
  • Online:2019-06-05 Published:2019-06-05
  • Contact: Prof. Peng Qinghua, Ph.D., Hunan University of Chinese Medicine, Hanpu Science and Technology Industrial Park, Yuelu District, Changsha City, Hunan Province, 410208. E-mail: pqh410007@126.com.

Abstract: Objective To observe the effects of Mimenghua (Flos Buddlejae) Granule (MG) on the expressions of IL-6 and IL-12 in lacrimal gland cells of castrated male rabbits.Methods Thirty New Zealand long-eared white rabbits were randomly and evenly divided into 5 groups: normal group, model group, MG group, saline group and testosterone group. Except rabbits in the normal group, all the others were castrated by bilateral orchiectomy and epididymectomy. SIT and BUT test were performed 1 day before and 30 days after castration. After 30 days of intervention, all rabbits were executed. Lacrimal gland tissues were collected for HE staining to observe the structure of lacrimal gland. Immunohistochemistry was used to detect the expressions of IL-6 and IL-12 in lacrimal gland tissues from rabbits of all groups.Results In the normal group, the lacrimal gland tissue was neatly arranged and the structure was clear. No expression of IL-6 and IL-12 was observed. In the model group and the saline group, the lacrimal gland tissue was not aligned, and the structure was degenerated. IL-6 and IL-12 were expressed in the cell membrane and in the cytoplasm. The lacrimal gland tissue of the MG group and the testosterone group were neatly arranged, and the structure was clear. Only small amounts of IL-6 and IL-12 were expressed in the cell membrane and cytoplasm. Compared with the normal group, the SIT and BUT values in the model group and saline group were decreased (P<0.05), and the differences in SIT and BUT values in the MG group and testosterone group were not statistically significant (P>0.05).Conclusion MG can reduce inflammation of lacrimal gland tissues by inhibiting the expression of IL-6 and IL-12 in lacrimal gland tissues to treat dry eyes.

Key words: Mimenghua Granule, lacrimal gland tissues, inflammatory factor, castration, rabbit

CLC Number: 

  • R285.5