主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (6): 496-501.doi: 10.3969/j.issn.1006-2157.2019.06.011

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of “Heart-kidney therapy” on retinal neurotrophic factor in diabetic rats*

Wu Zhengzheng1, Wang Qian2, Ge Dongyu2, Yan Jing1#, Gong Ximei1, Liu Huixian1   

  1. 1 Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100040, China;
    2 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Online:2019-06-05 Published:2019-06-05
  • Contact: Yan Jing, Ph.D., Deputy Chief Physician. CPC Committee Office, Eye Hospital, China Academy of Chinese Medical Sciences, No.33 Lugu Road, Shijingshan District, Beijing, 100040. E-mail: yjfly8377@sina.com

Abstract: Objective To explore the protective mechanism of “Heart-kidney therapy” for retinal nerve dysfunction in diabetic rats.Methods SD rats were randomly divided into normal group, model group and Mimenghua (Flos Buddlejae) compound prescription (MCP) group. Diabetic rat models were created by intraperitoneal injection of streptozotocin (STZ). Intragastric administration of MCP was carried out for 90 days consecutively. Visual electrophysiology was used to detect the visual function of diabetic rats on 30, 60, 90 d respectively. The morphological changes of retina were observed with HE staining on 90 d. Apoptosis of retinal ganglion cells was detected with TUNEL. The mRNA and protein expression of brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) in retina were detected with PCR and Western blot.Results Visual electrophysiology examination showed that the OPs wave amplitude and b-wave amplitude in ERG were decreased which indicated decreased optic nerve function in the model group with statistically significant difference, compared with the normal group (P<0.01). HE staining showed that retinal ganglion cells in the model group were swollen, disordered and decreased in number. PCR and Western blot showed that the mRNA and protein expression of BDNF and CNTF were decreased, compared with the normal group. After the intervention with the Chinese prescription (Mimenghua prescription), the OPs wave amplitude and b-wave amplitude in ERG were increased in the MCP group, compared with the model group (P<0.01). The number of apoptotic retinal ganglion cells decreased, and mRNA and protein expression of BDNF and CNTF in retina increased in the MCP group, compared with the model group.Conclusion Mimenghua compound prescription can protect the retinal nerve function in diabetic rats by regulating the expression levels of mRNA and protein of BDNF and CNTF.

Key words: Mimenghua compound prescription, diabetes mellitus, electrophysiology, BDNF, CNTF, rats

CLC Number: 

  • R285.5