主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (7): 584-591.doi: 10.3969/j.issn.1006-2157.2019.07.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Tibetan medicine Tinospora sinensis on the expression of inflammatory cytokines and NMDA receptors in the spinal cord of rats with pain-sensitive hyperinjury*

Ma Dan, Zhang Shuofeng#, Li Yinghui, Cheng Wenhao, Song Jingyi, Cheng Long   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2019-01-25 Online:2019-07-30 Published:2019-09-03
  • Contact: Prof. Zhang Shuofeng, Ph.D., Master tutor. Beijing University of Chinese Medicine, School of Chinese Medicine, Beijing 102488. E-mail: shuofengzhang@sina.com

Abstract: Objective To observe the effect of water extract of Tinospora sinensis on hypersensitivity to temperature and mechanical stimulation in rats with inflammatory pain and its anti-inflammatory and analgesic mechanism. The expression levels of IL-1α in serum, IL-6, IL-10, TNF-α and N-methyl-D-aspartic acid (NMDA) receptors in the spinal dorsal horn of the affected side were measured to explore the anti-inflammatory and analgesic mechanisms of Tinospora sinensis in the treatment of inflammatory pain in rats. Methods Rats were randomly divided into five groups by using randomized sequence table: normal group, model group, prednisone acetate group, high-dose, and mid-dose Tinospora sinensis water extract (TSWE) groups. After 24 hours of Freund’s adjuvant injection to establish the inflammatory model, the rats were intragastrically administered for 12 days. The reaction value of 50% mechanical foot contraction pain, reaction time of thermal foot contraction and swelling rate of affected foot and sole were then evaluated. After 1 day of the last administration, the rats were sacrificed with feet perfused and fixed. ELISA method was used to determine the expression of IL-1α, IL-6, IL-10 and TNF-α. The spinal cord was taken out for immunohistochemistry to observe the expression of NMDA receptor in the dorsal horn of the affected side. Results Compared with the control group, rats in the model group developed significant swelling in the ankle and feet (P<0.01). The 2.4 g/kg Tinospora sinensis water extract significantly reduced the degree of swelling at day 12 of administration (P<0.01, compared with the model group, P<0.05). The reaction time of heat-shrinking foot in model rats was significantly reduced and there was significant difference between the two groups (P<0.01, compared with the control group). Different doses of Tinospora sinensis could obviously increase the reaction time of rat’s heat-shrinking foot and prolong the pain-sensitive reaction of affected limbs at different time points, which had statistical significance (P<0.01, compared with the model group). The 50 % mechanical pain response value of the model rats was significantly lower than that of control group (P<0.01). The analgesic effect of Tinospora sinensis group lasted for 12 days, which was significantly different from that of the normal group (P<0.01). Compared with the normal group, serum inflammatory cytokines IL-1α, TNF-α and IL-6 in the model rats were significantly increased (P<0.01), and IL-10 was significantly decreased (P<0.01). After 12 days of administration, Tinospora sinensis water extract could significantly reduce the IL-1α, TNF-α and IL-6 and increase the IL-10 in the model rats (P<0.01, compared with the normal group). Compared with the normal group, the expression of NMDA receptor in the spinal dorsal horn of the injured side of the inflammatory pain model rats increased (P<0.01). Compared with the model group, 1.2, 2.4 g/kg Tinospora sinensis water extract could significantly reduce the expression of NMDA receptor in the spinal cord of rats with inflammatory pain model (P<0.01). Conclusion Tinospora sinensis water extract can effectively reduce the high sensitivity of temperature and mechanical stimulation in rats with inflammatory pain, and its anti-inflammatory and analgesic mechanism is related to reducing the production of peripheral inflammatory factors and inhibiting the expression of NMDA receptors.

Key words: Tinospora sinensis, inflammatory pain, NMDA, inflammatory factors, rats

CLC Number: 

  • R285.5