主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (8): 655-661.doi: 10.3969/j.issn.1006-2157.2019.08.008

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Influence and mechanism of Danshen Tongluo Jiedu Tang combined with bone marrow stem cell transplantation on myocardial fibrosis in rats with acute myocardial infarction*

Li Xinhui, Huang Miaoxin, Du Jianfang, Chen Cong, Yang Yanhong, Wu Ruoxia, Wang Jingwen, Li Caiyun   

  1. School of Chinese Medicine, Hunan University of Chinese Medicine, Hunan 410208, China
  • Received:2019-02-15 Online:2019-08-30 Published:2019-09-04
  • Contact: Pro. Li Xinhui, Ph. D., Master’s supervisor. School of Chinese Medicine, Hunan University of Chinese Medicine, No. 300, Xueshi Road, Hanpu Science and Education Park, Yuelu District, Changsha 410208. E-mail: 3077314414@qq.com

Abstract: Objective To investigate the influence and mechanism of Danshen Tongluo Jiedu Tang (Salvia Root Collateral-freeing Removing Toxicity Decoction) combined with bone marrow stem cell transplantation (BMSCT) on myocardial fibrosis in rats with acute myocardial infarction (AMI). Methods The bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured by using whole bone marrow adherence method combined with density gradient centrifugation. The model of AMI was established through ligating the left anterior descending branch (LAD) of coronary artery. The rats were randomly divided into sham-operation group, AMI model group, BMSCT group, Danshen Tongluo Jiedu Tang group (Chinese medicinal group) and Danshen Tongluo Jiedu Tang+BMSCT group (combination group). The model was established in all groups except of sham-operation group, and then Chinese medicinal group and combination group were respectively given orally DTJT and other groups were given orally equivalent saline water once a day for 14 days continuously. The content of collagen in myocardial tissue was observed after Masson staining, and concentration of procollagen type III N-terminal peptide (PⅢP), procollagen type IV N-terminal peptide (PIVP), nitric oxide (NO) and endothelin-1 (ET-1) were detected by using ELISA. The expressions of endothelial protein C receptor (EPCR) and thrombomodulin (TM) were detected by using Western blotting assay, and all data was analyzed by using SPSS 19.0 statistical software. Results Compared with sham-operation group, the protein expressions of EPCR and TM decreased, NO level decreased, levels of ET-1, PⅢP and PⅣP increased, and myocardial collagen fiber generation in varying degrees was observed in other groups (P<0.01). The protein expressions of EPCR and TM and NO level increased, and levels of ET-1, PⅢP and PⅣP and myocardial collagen fiber generation decreased in BMSCT group, Chinese medicinal group and combination group compared with AMI group (P<0.01). The protein expressions of EPCR and TM and NO level increased, and levels of ET-1, PⅢP and PⅣP and myocardial collagen fiber generation decreased in Chinese medicinal group compared with BMSCT group (P<0.01). The protein expressions of EPCR and TM and NO level increased, and levels of ET-1, PⅢP and PⅣP and myocardial collagen fiber generation decreased in combination group compared with Chinese medicinal group (P<0.01). Conclusion Danshen Tongluo Jiedu Tang combined with BMSCT can inhibit myocardial fibrosis, and the mechanism may be related to that the protein expressions of myocardial EPCR and TM are up-regulated and vascular endothelial function is protected in AMI rats.

Key words: acute myocardial infarction, endothelial protein C receptor, thrombomodulin, bone marrow stem cells, Danshen Tongluo Jiedu Tang (Salvia Root Collateral-freeing Removing Toxicity Decoction), procollagen type III N-terminal peptide, procollagen type IV N-terminal peptide, rats

CLC Number: 

  • R285.5