主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (8): 662-666.doi: 10.3969/j.issn.1006-2157.2019.08.009

• Science & Technology Theme • Previous Articles     Next Articles

Diosgenin’s inhibitory effects on proliferation and migration of MCF-7 breast cancer cells through demethylation of microRNA-145*

Li Ya, Lyu Peng, Hou Li, Zhang Yayue, Wang Chong, Fan Qiuyue, Chen Xinyi, Shi Fengqin#   

  1. Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2019-04-12 Online:2019-08-30 Published:2019-09-04
  • Contact: Shi Fengqin, Ph. D., Attending Physician. Dongzhimen Hospital, Beijing University of Chinese Medicine. No. 5, Haiyuncang Street, Dongcheng District, Beijing 100700. E-mail: shifengqinbj@sina.com.

Abstract: Objective To discuss the effect and possible mechanism of diosgenin on MCF-7 breast cancer cells. Methods Human normal breast epithelial cell line MCF-10A and human breast cancer cell line MCF-7 were chosen, and diosgenin in different concentrations was added in the cells. The influence of diosgenin on proliferation of MCF-7 breast cancer cells was detected by using MTS assay. The influence of diosgenin on migration ability of MCF-7 breast cancer cells was detected by using wound-healing test. The effect of diosgenin on methylated microRNA-145 (miR-145) of MCF-7 breast cancer cells was detected by using methylation-specific polymerase chain reaction (MSP). The expression of miR-145 mRNA of MCF-7 breast cancer cells was detected by using quantitative real-time polymerase chain reaction (qPCR). Results Diosgenin inhibited significantly the proliferation of MCF-7 breast cancer cells in a concentration-and time-dependent manner, and proliferation inhibitory rate for MCF-7 breast cancer cells was significantly higher than that for normal mammary epithelial cells MCF-10A (P<0.05). Diosgenin reduced significantly the migration ability of MCF-7 breast cancer cells in a concentration-dependent manner (P<0.01). Compared with control group, miR-145 showed a demethylation state, and expression of miR-145 mRNA increased significantly (P<0.01), which was positively correlated to diosgenin concentration in diosgenin group. Conclusion Diosgenin can inhibit the proliferation and migration of breast cancer cells, and the mechanism may be related to the regulation of expression and methylation of miR-145.

Key words: diosgenin, breast cancer, microRNA-145, demethylation

CLC Number: 

  • R285.5