主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (9): 766-772.doi: 10.3969/j.issn.1006-2157.2019.09.011

• Chinese Medicinal Chemistry • Previous Articles     Next Articles

High-speed countercurrent chromatography based on screening of new solvent systems in separation of polyphenols compounds from olive leaves*

Wang Yu1,2, Xia Pengfei1, Yang Xue1, Duan Wenda1, Quan Kaijun2, Huang Xinyi2, Di Duolong1,2, Zhao Lei1#   

  1. 1 Gansu University of Chinese Medicine, Gansu 730000, China;
    2 Key Laboratory of Chemistry of Northwestern Characteristic Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Gansu 730000, China
  • Received:2019-03-28 Online:2019-09-30 Published:2019-09-27
  • Contact: Prof. Zhao Lei, Ph. D., Doctoral Supervisor. Gansu University of Chinese Medicine. No. 35 Dingxi East Road, Chengguan District, Lanzhou 730000. E-mail: zzyhx@gszy.edu.cn
  • Supported by:
    National Natural Science Youth Foundation (No. 21505144), Open Fund Project of Engineering Laboratory for Research and Promotion of Quality Standardization Technology of Authentic Medicinal Substances in Gansu Province(No.ddyc-2016-04), Research Project Funded by Gansu Provincial Administration of Traditional Chinese Medicine (No.G2KLP-2014-7, No.G2K-2014-77)

Abstract: Objective A novel 9×9 map-based solvent selection strategy for a system composed of hexane-ethyl acetate-methanol-water (HEMW) was applied to high-speed countercurrent chromatography (HSCCC) separation of polyphenols oleuropein, luteolin-4'-O-β-D-glucoside and hydroxytyrosol from olive leaf extracts. Methods The sweet solvent system HEMW (1.2∶8.8∶1.7∶8.3,V/V) was selected by determining the distribution coefficient K values of the target compounds in four different solvent systems and establishing linear relationships between the Log K values and the number of solvent systems. The sweet solvent system HEMW (1.2∶8.8∶1.7∶8.3, V/V) was then used to separate olive leaf extracts by HSCCC, and the parameters of flow rate, rotational speed and injection volume were optimized. Results 45.8 mg oleuropein, 17.7 mg luteolin-4'-O-β-D-glucoside and 6.8 mg hydroxytyrosol were obtained from 500 mg olive leaf extracts by using the sweet solvent system HEMW (1.2∶8.8∶1.7∶8.3, V/V) with flow rate at 1.5 mL/min, rotation speed at 950 r/min and injection concentration at 20 g/L, and the purity of each of the three compounds obtained was more than 90%. Conclusion This study demonstrates the feasibility and efficiency of 9×9 map-based solvent selection strategy in the field of HSCCC separation of natural products, and provides technical support for rapid screening of HSCCC solvent system and improving the efficiency of HSCCC separation and preparation of target compounds in complex systems.

Key words: new solvent system, HSCCC, olive leaves, separation

CLC Number: 

  • R284.1