主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (10): 847-853.doi: 10.3969/j.issn.1006-2157.2019.10.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of Xiefei Decoction on the inflammatory factors and VEGF expression in rats with pulmonary fibrosis*

Yan Chunlu1, An Fangyu1,2,3#, Liu Yongqi1,2,3, Luo Yali1, Wu Zhiwei1, Liu Xuesong1, Li Yang1, Shi Xufeng1, Zhang Xue1   

  1. 1 Gansu University of Chinese Medicine, Gansu 730000, China;
    2 Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Gansu 730000, China;
    3 Key Laboratory of Dunhuang Medicine, Ministry of Education, Gansu 730000, China
  • Received:2019-04-10 Online:2019-10-30 Published:2019-11-25
  • Contact: A/Prof. An Fangyu, MA, Teaching and Experimental Training Center, Gansu University of Chinese Medicine, Gansu 30000, China. E-mail: anfyuwsmh@ 163.com
  • Supported by:
    Natural Science Foundation Project of Gansu Science and Technology Department (No.148RJZA065), the Open Fund Project of Key Laboratory of medicinal chemistry and quality research(No.DHYX1213-013), Project of Improving Employment and Entrepreneurship Abillity of College Students of Gansu Province(No.6-1)

Abstract: ObjectiveTo observe the effect of Xiefei Decoction (Lung Heat-clearing Decoction, XFT) on the inflammatory factors and the expression of VEGF in lung tissue of pulmonary fibrosis rats induced by bleomycin. Methods 84 SPF SD rats were randomly divided into normal group, model group, XFT low, middle and high-dose XFT groups and prednisone group (n = 14 in each group). Apart from the normal group, a single dose of bleomycin was intratracheally injected into the SD rats to induce pulmonary fibrosis (PF). The normal group received the same amount of normal saline. After 24 hours, low, middle and high-dose XFT groups were given a gavage of 10 g/kg·d, 20 g/kg·d and 40 g/kg·d respectively; the prednisone group was given prednisone 1.8 mg/kg·d; and the normal group and model group were given equal volume of normal saline. All rats were sacrificad after 28 days. The pathomorphism changes of lung tissue were observed with HE; the contents of IL-6 and IL-10 in serum were evaluated with ELISA; expression of VEGF gene and caspase-3 gene in lung tissue were assessed by RT-PCR; and VEGF expression in lung tissue was assessed by Western blot. Results Compared with the blank control group, the pathological score, inflammatory cell count, serum IL-6 content, and the expression of caspase-3 and VEGF in lung tissue were significantly increased, but serum IL-10 was significantly decreased in the model group (P<0.01). Compared with the model group, in all XFT groups, the expression of VEGF decreased significantly; and the pathological score, inflammatory cell count, serum IL-6 contents, the gene expression of caspase-3 and VEGF were decreased significantly (P<0.05 or P<0.01). However, the serum IL-10 content was significantly increased in XFT mid-dose and high-dose groups compared with the model group (P<0.05 or P<0.01). Conclusion XFT seems to delay disease progression of pulmonary fibrosis, possibly through inhibition of inflammation, apoptosis and angiogenesis.

Key words: Xiefei Decoction (Lung Heat-clearing Decoction), pulmonary fibrosis, inflammatory factors, VEGF

CLC Number: 

  • R285.5