主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (11): 916-922.doi: 10.3969/j.issn.1006-2157.2019.11.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Mechanism of Huotan Jiedu Tongluo Fang in intervening endoplasmic reticulum stress-autophagy signaling pathway in rabbits with carotid atherosclerosis*

Yu Keying1, Deng Yue1#, Shi Rui2, Zhao Mingjing2, Shen Juanjuan1, Gao Ling3, Tian Tenghui1, Niu Wang3, Wang Chunmei3, Xue Wen3, Zhao Yue3   

  1. 1 Affiliated Hospital of Changchun University of Chinese Medicine, Jilin 130021, China;
    2 Education Ministry and Beijing Key Laboratory of Chinese Internal Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    3 Changchun University of Chinese Medicine, Jilin 130117, China
  • Received:2018-11-29 Online:2019-11-30 Published:2019-12-03
  • Contact: Deng Yue, Ph. D., Chief Physician, Doctoral supervisor. Affiliated Hospital of Changchun University of Chinese Medicine, No. 1478, Gongnong Dalu Road, Chaoyang District, Jilin 130021. E-mail: dyue7138@sina.com

Abstract: Objective To study the effect of Huotan Jiedu Tongluo Fang (HTJDTLF, Sweeping Phlegm-Detoxicating-Freeing Collateral Vessels Formula) on intervening endoplasmic reticulum stress (ERS)-autophagy signaling pathway in rabbits with carotid atherosclerosis (CAS). Methods Male Japanese rabbits aged 10 weeks old were randomly divided into sham-operation group, model group, HTJDTLF group and atorvastatin group. CAS model was established through feeding methionine fodder and balloon injury of common carotid artery intima. The samples of injured common carotid artery were collected and given HE staining for observing histopathological structure. The content of serum homocysteine (Hcy) was detected by using ELISA in all groups. The mRNA levels of protein kinase-like endoplasmic reticulum kinase (PERK, an ERS marker molecule), eukaryotic initiation factor 2ɑ (eIF-2ɑ) and autophagy marker molecule-Atg5 in common carotid artery tissue were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The protein expressions of eIF-2ɑ and Atg5 in common carotid artery tissue were detected by using Western blotting assay. Results Compared with sham-operation group, damaged common carotid artery had significant intimal hyperplasia and vascular lumen stanosis, and the content of serum Hcy increased (P<0.01) in model group. Compared with model group, the intimal hyperplasia was significantly relieved and content of serum Hcy decreased in HTJDTLF group (P<0.01). The mRNA levels of PERK, eIF-2ɑ and Atg5 and protein expressions of eIF-2ɑ and Atg5 increased in model group compared with sham-operation group (P<0.01). The mRNA levels of PERK, eIF-2ɑ and Atg5 decreased (P<0.01) and protein expressions of eIF-2ɑ and Atg5 decreased (P<0.01) in HTJDTLF group and atorvastatin group. Conclusion HTJDTLF can significantly relieve CAS and reduce serum Hcy content in rabbits with CAS, and the mechanism maybe related to the inhibition of excessive activation of ERS-autophagy signaling pathway

Key words: sweeping phlegm, detoxicating and freeing collateral vessels, carotid atherosclerosis, endoplasmic reticulum stress, autophagy, rabbits

CLC Number: 

  • R285.5