主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (11): 934-939.doi: 10.3969/j.issn.1006-2157.2019.11.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Influence of medicated serum of Houshi Hei San on expressions of PLCγ2/ERK and TGF-β/Smad2/3 in vascular endothelial cells after injury of oxygen-glucose deprivation*

Xiang Yangyang, Wang Xuan, Zhang Qiuxia#   

  1. Beijing Key Laboratory of Research on Collateral Disease of Chinese Medicine, School of Traditional Chinese Medicine, Capital University of Medical Sciences, Beijing 100069, China
  • Received:2019-05-23 Online:2019-11-30 Published:2019-12-03
  • Contact: Prof. Zhang Qiuxia, Ph.D., Doctoral supervisor. School of Traditional Chinese Medicine, Capital University of Medical Sciences, No. 10, Youanmenwai Xitoutiao, Fengtai District, Beijing 100069. E-mail: zqx26@163.com

Abstract: Objective To investigate the influence of medicated serum of Houshi Hei San (Hou’s Black Powder, HHS-MS) on expressions of phospholipase Cγ2 (PLCγ2), extracellular regulated kinase (ERK), transforming growth factor-β (TGF-β) and Smad2/3 in human umbilical vein endothelial cells (HUVEC) after injury of oxygen-glucose deprivation (OGD). Methods HUVEC were randomly divided into control group, model group, 2.5% HHS-MS group, 5.0% HHS-MS group, 10.0% HHS-MS group and 20.0% HHS-MS group. The OGD model was established through putting HUVEC in a hypoxic incubator for continuous cultivation for 6 h after changed to sugar-free nutrient solution in model group and all HHS-MS groups. HUVEC were cultured normally in the same duration. After modeling, the nutrient solution was changed to complete nutrient solution with 10% blank serum in control group and model group, and MS with corresponding mass fraction of HHS-MS was used in all HHS-MS groups. The HUVEC total protein was extracted after normal cultivation for 24 h. The protein expressions of PLCγ2, ERK, p-ERK, TGF-β and Smad2/3 in HUVEC were detected by using Western blotting assay. Results Compared with control group, the expression of PLCγ2 and Smad2/3 were up-regulated (P<0.01) and expression of TGF-β was down-regulated (P<0.01) in model group. Compared with model group, the expression of TGF-β was up-regulated in 5.0% HHS-MS group and expressions of PLCγ2 and Smad2/3 were down-regulated in 2.5% HHS-MS group, 5.0% HHS-MS group and 10.0% HHS-MS group (P<0.01 or P<0.05). Conclusion HHS-MS can regulate PLCγ2/ERK signaling pathway and TGF-β/Smad2/3 signaling pathway, and improve the proliferation of HUVEC after OGD injury.

Key words: Houshi Hei San(Hou’s Black Powder), oxygen-glucose deprivation, phospholipase Cγ2/extracellular regulated kinase, transforming growth factor, Smad2/3, human umbilical vein endothelial cells

CLC Number: 

  • R285.5