主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (12): 1024-1029.doi: 10.3969/j.issn.1006-2157.2019.12.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of Inula britannica flower total flavonoids on autophagy of aging L929 cells*

Guo Zongyu, Liu Lu, Kuang Jie, Zhou Maoqiang, Guo Lei#   

  1. Department of Burn and Plastic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Received:2019-06-19 Online:2019-12-30 Published:2020-01-06
  • Contact: Associate Prof. Guo Lei, Ph.D., Master’s Supervisor. The First Affiliated Hospital of Chongqing Medical University. No.1, Youyi Road, Yuanjiagang, Yuzhong District, Chongqing 400016. E-mail:841602937@qq.com

Abstract: Objective To investigate the effect of Inula britannica flower total flavonoids (IBFTF) on autophagy of aging L929 cells by detecting the levels of autophagy in the process of senescence of L929 cells for better development of the medicinal value of IBFTF. Methods Cell senescence models were established by using D-galactose (D-gal) solutions (0, 5, 10, 20 and 40 g/L) on L929 cells to find out the most appropriate concentration of D-gal for inducing senescence in L929 cells. IBFTF solutions (25, 50, 100 mg/L) were applied to aging L929 cells to investigate the effect of IBFTF on L929 cell senescence and autophagy. The effect of D-gal on the viability of L929 cells was detected with MTT. β-galactosidase staining was used to detect the effect of D-gal at different concentrations on the senescence process of L929 cells. Fluorescence microscopy was used to detect the effect of IBFTF on ROS production in senescent L929 cells. The effects of IBFTF on the number of lysosomes and the expression of LC3 protein in senescent L929 cells were examined with acridine orange staining and immunofluorescence. The levels of autophagy-related proteins were detected with Western-Blot. Results IBFTF delayed the senescence process of aging L929 cells in a dose-dependent manner, and the autophagy level of L929 cells also increased in a dose-dependent manner during the aging delay process. Conclusion IBFTF delayed the senescence of D-gal-induced senescent cells, which may be related to the elevated levels of autophagy.

Key words: Inula britannica flower total flavonoids, L929 cells, autophagy, aging

CLC Number: 

  • R285.5