主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2019, Vol. 42 ›› Issue (12): 1043-1048.doi: 10.3969/j.issn.1006-2157.2019.12.012

• Clinical Studies • Previous Articles     Next Articles

Investigation of differences between cold and heat syndromes of bronchial asthma based on biomarker of small molecular metabolites*

Liu Shigang, Li Meng, Zhang Jiuqing, Zhang Xia, Liu Li, Li Hui#   

  1. Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
  • Received:2019-06-14 Online:2019-12-30 Published:2020-01-06
  • Contact: Li Hui, Ph.D., Chief Physician, Doctoral Supervisor. Guang’anmen Hospital, China Academy of Chinese Medical Sciences. No.5, Beixian’ge, Xicheng District, Beijing 100053. E-mail:hui_li269@sina.com

Abstract: Objective To explore the difference in small molecular metabolites between deficiency cold syndrome and deficiency heat syndrome of chronic bronchial asthma and the correlation between the clinical syndromes and the metabolites to identity potential biomarkers that could differentiate asthma syndromes based on the metabonomics. Methods Thirty subjects (ten of deficiency cold syndrome, ten of deficiency heat syndrome, and ten healthy volunteers) were recruited in the study. With informed consent from the subjects, 2 mL of blood was collected containing anticoagulant and kept in a static and anticoagulant condition, and then serum was separated by centrifugation and stored at -80 ℃ in refrigerator. It was analyzed with GC-MS and multidimensional analysis (O) PLS-DA and one-dimensional analysis (t test) were made to identify different metabolites between the groups (VIP>1, P<0.05). Then enrichment analysis of pathways of differential metabolites was done with MBRole and KEGG. Results Forty different metabolites were found between chronic asthma patients and healthy controls, and eighteen such metabolites between patients of cold and heat deficiency syndromes. Among them, four small molecular metabolites can tell both asthma patients from healthy controls and deficiency cold syndrome from deficiency heat syndrome, i.e. tetracosane, 4-aminobutyric acid, benzoylformic acid, and allo-Inositol. MBRole and KEGG pathway analysis of the metabolites of the deficiency cold syndrome group and deficiency heat syndrome group found disturbances of pathways of beta-alanine metabolism. Conclusion The small molecular metabolites are significantly different between chronic bronchial asthma patients and healthy controls, and between patient of cold deficiency syndrome and those of heat deficiency syndrome. 4-aminobutyric acid might be the potential biomarkers to differentiate deficiency cold syndrome from deficiency heat syndrome of chronic bronchial asthma patients, and beta-alanine metabolic pathway disturbance was found between deficiency cold syndrome group and deficiency heat syndrome group.

Key words: bronchial asthma, chronic, metabonomics, deficiency cold syndrome, deficiency heat syndrome

CLC Number: 

  • R256.12