主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (1): 43-49.doi: 10.3969/j.issn.1006-2157.2020.01.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of resveratrol on the proliferation of rat bone marrow-derived endothelial progenitor cells*

Yu Huizhen1, Chen Cheng2, Chen Junming1, Zhu Pengli1#   

  1. 1 Fujian Provincial Clinical Medical College, Fujian Medical University, Fuzhou 350001, China;
    2 The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China
  • Received:2019-08-16 Published:2020-02-21
  • Contact: Prof. Zhu Pengli, Chief Physician, Doctoral Supervisor. Fujian Provincial Clinical Medical College, Fujian Medical University, Fujian Institute of Clinical Geriatrics, No.134, East Road, Fuzhou 350001, China. E-mail: zhupengli7755@163.com
  • Supported by:
    National Natural Science Foundation of China (No.81670258, No.81873515, No.81373785), Natural Science Foundation of Fujian (No.2017J01247)

Abstract: Objective We used isolation of density gradient centrifugation and culture on rat bone marrow-derived endothelial progenitor cells (EPCs) to observe the effect of resveratrol on the proliferative capacity of EPCs. Methods The rat bone marrow-derived mononuclear cells were isolated by using density gradient centrifugation. Nonadherent cells were removed. Adherent cells were cultured for 14 days to obtain EPCs. The cell markers of CD31, vWF, FLK-1, and CD45 were measured by using immunofluorescence. The uptake of Dil-Acetylated Low Density Lipoprotein (DiL-AC-LDL) and FITC-Ulex europaeus agglutinin-1(FITC-UEA-1) and the Matrigel tube formation assay were conducted to identify EPCs. The influence of resveratrol at different concentrations(5, 10, 25, 50, 100 μmol/L)for 48 h and at different time periods (24~72 h) of culture on the proliferative capacity of EPCs were detected by using CCK8 assay. Results Isolated bone marrow derived-mononuclear cells with a cobblestone and pavement stone appearance were cultured after 2 weeks. The immunofluorescence demonstrated that more than 90% cells expressed CD31, vWF and FLK-1. Moreover, the cells could take in DiI-AC-LDL and FITC-UEA-1. In addition, these cells could develop well-formed tube networks when plated in matrigel. According to the morphological, immunophenotypic and functional characteristics, the cells in the study were verified to be rat EPCs. Compared with the control group, after incubated with resveratrol for 48h, the EPCs’ proliferative capacity were improved in concentration (5~50 μmol/L) of resveratrol dependence(P<0.01 ). Compared with the control group, incubated with 50 μmol/L resveratrol for 24, 48 or 72 h, the EPCs’ proliferative capacity were improved in a time (24~72 h)-dependent way(P<0.05,<0.01,<0.01). Conclusion These cells isolated by density gradient centrifugation and detected by immunofluorescence were identified as rat bone marrow-derived endothelial progenitor cells. Furthermore, resveratrol could improve the proliferative capacity of rat bone marrow-derived endothelial progenitor cells. These may establish the foundation for the research in the cardiovascular protection mechanism of resveratrol in the future.

Key words: resveratrol, endothelial progenitor cells, proliferation, bone marrow, rat

CLC Number: 

  • R285.5