主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (1): 50-55.doi: 10.3969/j.issn.1006-2157.2020.01.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Jiawei Liu’an Jian on pathomorphology of airway remodeling in rats with cough variant asthma*

Zhang Ningning1, Wu Liqun2, Huo Jingwei2#, Zhu Ke1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2019-07-20 Published:2020-02-21
  • Contact: Huo Jingwei, Master, Attending Physician, Dongfang Hospital of Beijing University of Chinese Medicine, No.6, Fangzhuang, Fengtai District. Beijing 100078, China. E-mail: huo_jing_wei@126.com
  • Supported by:
    National Natural Science Foundation of China (No.81874487)

Abstract: Objective To observe the effects of Jiawei Liu’an Jian (Supplemented Six-Ingredient Decoction, JWLAJ) on airway inflammation and pathological changes of pulmonary tissue remodeling in rats with cough variant asthma (CVA), and to explore its mechanism in treating CVA, so as to provide evidence for clinical treatment. Methods A total of 60 Wistar rats were randomly allocated into normal group, model group, Montelukast sodium (MS) group, and treatment groups including low-dose, mid-dose and high-dose JWLAJ groups. The rats were sensitized with albumin and aluminium hydroxide, and then inhaled albumin to replicate CVA rat models. The infiltration of pulmonary inflammatory cells, the degree of collagen deposition (collagen area and collagen volume fraction) and morphological changes of airway in the lung tissues of rats in each group were observed. Results Compared with the normal group, inflammatory cell infiltration, hyperemia and edema could be observed in the lung tissues under the microscope in the model group. Compared with the model group, the amount of inflammatory cells in the lung tissues were reduced in all treatment groups, and the degrees of hyperemia and edema were also reduced. Collagen area: Compared with the normal group, the collagen area of the model group was increased (P<0.05); compared with each other in the treatment groups, the collagen area decreased most significantly (P<0.05) in the low-dose JWLAJ group; there was no statistically significant difference between the treatment groups and the MS group (P>0.05). Collagen volume fraction: Compared with the normal group, the collagen volume fraction of the model group was increased (P<0.05); compared with each other in treatment groups, the collagen volume fraction decreased most significantly (P<0.05) in the low-dose JWLAJ group; there was no statistically significant difference between the treatment groups and MS group (P>0.05). The airway morphological parameters: Compared with normal group, there was a significant increase in smooth muscle area/bronchial basement membrane diameter (Wam/Pbm), as well as airway wall area/bronchial basement membrane diameter (Wai/Pbm) in the model group (P<0.05). Compared within the treatment groups, the Wam/Pbm, Wai/Pbm ratios were the lowest in low-dose JWLAJ group (P<0.05). There was no statistically significant difference between the MS group and treatment groups (P>0.05). Conclusion JWLAJ could be used to prevent and treat CVA, probably by reducing infiltration, hyperemia and edema of inflammatory cells in lung tissues, reducing collagen deposition surrounding the airway, as well as interfering in airway remodeling.

Key words: Jiawei Liu’an Jian (Supplemented Six-Ingredient Decoction), cough variant asthma, inflammatory cells, airway remodeling, rats

CLC Number: 

  • R285.5