主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (2): 108-114.doi: 10.3969/j.issn.1006-2157.2020.02.004

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Anti-cancer effect of diosgenin through regulating miR-34a and its target genes in gastric cancer*

Li Ya, Li Ruibai, Shi Fengqin, Chen Xinyi, Lyu Liyuan, Zhao Huan, Li Xiaojie, Hou Li#   

  1. Department of Oncology and Hematology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2019-09-03 Published:2020-03-24
  • Contact: Hou Li, Ph.D., Chief Physician, Doctoral Supervisor. Dongzhimen Hospital, Beijing University of Chinese Medicine, No. 5, Haiyuncang, Dongcheng District, Beijing 100700, China. E-mail: houli1203@126.com
  • Supported by:
    National Natural Science Foundation of China (No. 81573959)

Abstract: Objective To investigate the inhibitory effect and its possible mechanism of diosgenin on the proliferation, colony formation, migration and invasion of human gastric cancer cell (AGS). Methods The cell proliferation assay was used to detect the inhibition effect of diosgenin on AGS proliferation. The colony formation assay was performed to detect the effect of diosgenin on the colony formation efficiency of AGS cells. The wound healing test and transwell chamber assay were used to detect the effect of diosgenin on the migration and invasion of AGS. The MicroRNA(MiRNA) Target Prediction was used to predict the target genes of miR-34a. The Quantitative real-time PCR(qPCR) method was performed to observe the effect of diosgenin on the expression of miR-34a, E2F1, E2F3 and CCND1 in AGS cells, and analyze the correlation between their expression and the prognosis of gastric cancer patients. Results Diosgenin with different concentrations (24 μmol/L,30 μmol/L,36 μmol/L,42 μmol/L,48 μmol/L)could significantly inhibit the proliferation of AGS cells, and there was a positive correlation between the inhibiting effect and the dose and time. In different concentrations of diosgenin (24 μmol/L,30 μmol/L,36 μmol/L,42 μmol/L,48 μmol/L), the colony formation efficiency of AGS cells was significantly decreased (P<0.01), and the migration and invasion of gastric cancer cells were significantly decreased (P<0.01), with the effect positively correlated with time and concentration.Diosgenin(36 μmol/L) significantly increased the expression of miR-34a in AGS cells and decreased the expression of E2F1, E2F3 and CCND1, which were target genes of miR-34a. The expression of E2F1, E2F3 and CCND1 genes was significantly negatively correlated with the prognosis of gastric cancer patients.Conclusion Diosgenin seems to inhibit the proliferation, migration and invasion of AGS cells partly by regulating miR-34a and down-regulating the expression of E2F1, E2F3 and CCND1 genes.

Key words: diosgenin, gastric cancer, miRNAs, target genes

CLC Number: 

  • R285.5